VERY RAPID ASSAY OF GAMMA-AMINOBUTYRIC ACID IN MOUSE-BRAIN REGIONS WITHIN 3 MINUTES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION
VERY RAPID ASSAY OF GAMMA-AMINOBUTYRIC ACID IN MOUSE-BRAIN REGIONS WITHIN 3 MINUTES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION
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DOI：
10.1016/0160-5402(89)90029-6
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发表时间：
1989-04-01
期刊：
影响因子：
--
通讯作者：
ITOH, T
中科院分区：
文献类型：
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作者：
MURAI, S;NAGAHAMA, H;ITOH, T
为了测定脑组织中的γ-氨基丁酸(GABA)，建立了一种快速、简便的高效液相色谱-电化学检测-邻苯二甲醛(OPA)柱前衍生化方法。将组织的高氯酸提取物与OPA/β-巯基乙醇溶液混合，并将混合物注入高效液相色谱系统。本方法允许在一次层析运行中在不到3分钟的时间内测定GABA。在本方法中，一个工作日可以分析多达100个样品。本方法已应用于氨基氧乙酸和异烟肼处理的小鼠不同脑区的GABA水平的测定。
For assay of gamma-aminobutyric acid (GABA) in brain tissue, a very rapid and simple chromatographic procedure using high-performance liquid chromatography (HPLC) with electrochemical detection (ECD) in combination with precolumn o-phthalaldehyde (OPA) derivatization is described. Perchloric acid extracts of the tissue were mixed with OPA/.beta.-mercaptoethanol solution and the mixture was injected onto the HPLC system. The present method permits GABA assay within less than 3 min in one chromatographic run. Up to 100 samples a working day can be analyzed in the present method. The present method has been applied to the measurement of GABA levels in discrete brain regions of mice treated with aminooxyacetic acid and isoniazid.