Ultrasensitive Immunosensor for Cardiac Troponin I Detection Based on the Electrochemiluminescence of 2D Ru-MOF Nanosheets.
Ultrasensitive Immunosensor for Cardiac Troponin I Detection Based on the Electrochemiluminescence of 2D Ru-MOF Nanosheets.
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DOI：
10.1021/acs.analchem.9b02169
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发表时间：
2019-06
影响因子：
7.4
通讯作者：
Mengxia Yan;Jing Ye;Qiuju Zhu;Liping Zhu;Jianshe Huang;Xiurong Yang
中科院分区：
文献类型：
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作者：
Mengxia Yan;Jing Ye;Qiuju Zhu;Liping Zhu;Jianshe Huang;Xiurong Yang
电化学发光（ECL）功能化的金属有机框架（MOFs）由于其多样性和可调的光学性质而在生物传感领域受到越来越多的关注。富羧基三（4，4 '-二羧酸-2，2'-联吡啶）钌（Ru（dcbpy）32+）是一种著名的电化学发光材料，具有良好的水溶性和优异的电化学发光性能，也有可能成为金属有机骨架的有机配体。本文采用一锅法在水溶液中合成了骨架中含有大量Ru（dcbpy）32+的功能化MOF纳米片（RuMOFNS）。在该方案中，Ru（dcbpy）32+作为有机配体与Zn（NO3）2中的Zn 2+配位，聚乙烯吡咯烷酮（PVP）作为结构导向剂控制片状结构的形成。为便于实际应用，以RuMOFNS为ECL探针，设计了一种“信号开启”的ECL免疫传感器，用于检测心肌肌钙蛋白I（cTnI）。该免疫传感器对cTnI的检测范围为1 fg/mL ~ 10 ng/mL，检出限为0.48 fg/mL，具有较高的灵敏度和良好的选择性。最后，将该传感器应用于人血清样本中cTnI的检测，结果令人满意，表明该传感器在生物分析和临床诊断中具有潜在的应用价值。
Electrochemiluminescence (ECL)-functionalized metal-organic frameworks (MOFs) have attracted increasing attention in biosensing in virtue of their diverse and tunable optical properties. A famous ECL luminophore, carboxyl-rich tris(4,4'-dicarboxylic acid-2,2'-bipyridyl) ruthenium(II) (Ru(dcbpy)32+), possesses the characteristics of good water solubility and excellent ECL performance and also has the potential to be the organic ligand of metal-organic frameworks. Herein, functionalized MOF nanosheets (RuMOFNSs) containing plenty of Ru(dcbpy)32+ in the frameworks were synthesized in aqueous solution by a simple one-pot method. In this protocol, Ru(dcbpy)32+ acted as organic ligand to coordinate with Zn2+ originated from Zn(NO3)2, and polyvinylpyrrolidone (PVP) was used as structure-directing agent to control the formation of sheetlike structure. For practical application, a "signal-on" ECL immunosensor was designed for cardiac troponin I (cTnI) detection by employing RuMOFNSs as ECL probe. The immunosensor exhibited high sensitivity and excellent selectivity for cTnI detection in the range from 1 fg/mL to 10 ng/mL with a detection limit as low as 0.48 fg/mL. Finally, the biosensor was successfully applied for the detection of cTnI in human serum sample with satisfactory results, demonstrating its potential application in bioanalysis and clinical diagnosis.