Optical dissection of stimulus-evoked retinal activation
Optical dissection of stimulus-evoked retinal activation
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DOI：
10.1364/oe.16.012446
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发表时间：
2008-08-18
期刊：
影响因子：
3.8
通讯作者：
Zhao, You-Bo
中科院分区：
文献类型：
--
作者：
Yao, Xin-Cheng;Zhao, You-Bo
Better understanding of stimulus-evoked intrinsic optical signals (IOSs) in the retina promises new methodology for study and diagnosis of retinal function. Using a flood-illumination near infrared (NIR) light microscope equipped with high-speed CCD (80 Hz) and CMOS (1000 Hz) cameras, we validated depth-resolved enface imaging of fast IOSs in isolated retina of leopard frog. Both positive (increasing) and negative (decreasing) IOSs were observed at the photoreceptor and inner layers of the retina. The distribution of IOSs with opposite polarities showed a center-surround pattern. At the photoreceptor layer, negative IOSs dominated the center area illuminated by the stimulus light spot, while positive signals dominated the surrounding area. In contrast, at inner retinal layers, positive IOSs dominated the center area covered by the stimulus light spot, and negative IOSs were mainly observed in the surrounding area. Fast CMOS imaging disclosed rapid IOSs within 5 ms after the stimulus onset, and both ON and OFF optical responses were observed associated with a step light stimulus. (c) 2008 Optical Society of America.
