Short-term functional and neuroregenerative response of the urethra to ovariectomy and vaginal distension in female rats
Short-term functional and neuroregenerative response of the urethra to ovariectomy and vaginal distension in female rats
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DOI：
10.1007/s00192-004-1237-6
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发表时间：
2005-03-01
影响因子：
1.8
通讯作者：
Damaser, MS
中科院分区：
文献类型：
--
作者：
Ferguson, CL;Lin, DL;Damaser, MS
本研究旨在探讨卵巢切除（OVX）和阴道扩张（VD）对雌性大鼠漏尿点压力（LPP）和阴部神经再生反应的影响。20只大鼠在VD或假扩张前3天接受OVX。17只大鼠未接受OVX，但进行VD或假扩张。扩张后4天，进行LPP测试。在阴部神经的运动神经元上进行β（II）微管蛋白mRNA的原位杂交，神经再生反应的指标。在非OVX组中，VD后LPP显著降低。OVX后，VD和假手术大鼠之间的LPP差异尚未完全达到统计学显著性水平。OVX和VD对LPP的影响之间存在统计学显著的相互作用。任何组之间的原位杂交结果均无显著差异。VD或OVX后均未见阴部神经运动神经元的神经再生反应。
The objective of this study was to investigate the effects of ovariectomy (OVX) and vaginal distension (VD) on leak point pressure (LPP) and pudendal nerve regenerative response in the female rat. Twenty rats underwent OVX 3 days prior to either VD or sham distension. Seventeen rats did not receive OVX but underwent either VD or sham distension. Four days after distension, LPP testing was performed. In situ hybridization for beta(II) tubulin mRNA, an indicator of the neuroregenerative response, was performed on motoneurons of the pudendal nerve. In the non-OVX group, LPP was significantly decreased after VD. After OVX, the difference in LPP between VD and sham rats did not quite reach the level of statistical significance. There was a statistically significant interaction between the effects of OVX and VD on LPP. There was no significant difference in in situ hybridization results between any of the groups. No neuroregenerative response of motoneurons of the pudendal nerve was observed after either VD or OVX.