Resveratrol inhibits hepatoma cell invasion by suppressing gene expression of hepatocyte growth factor via its reactive oxygen species-scavenging property
Resveratrol inhibits hepatoma cell invasion by suppressing gene expression of hepatocyte growth factor via its reactive oxygen species-scavenging property
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DOI：
10.1007/s10585-004-2698-1
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Yagasaki, K
中科院分区：
文献类型：
--
作者：
Miura, D;Miura, Y;Yagasaki, K
白藜芦醇是一种多酚类化合物，存在于葡萄和其他植物中，具有很强的抗氧化活性。在我们之前的研究中。我们发现活性氧（ROS）加速了培养的大鼠腹水肝癌细胞系AH109A的侵袭能力，白藜芦醇和负载白藜芦醇的大鼠血清抑制了ROS增强的肝癌细胞的侵袭能力。为了研究白藜芦醇及其体内代谢物抑制入侵的机制，我们在AH109A细胞中估计了细胞内过氧化水平和肝细胞生长因子（HGF）的表达，HGF是一种已知的细胞运动因子。外源添加ROS可促进细胞内过氧化物水平和HGF的表达。白藜芦醇和负载白藜芦醇的大鼠血清消除了ros刺激肿瘤细胞中过氧化氢水平和HGF表达的升高。这些结果表明，白藜芦醇的抗氧化特性和口服白藜芦醇的大鼠血清对ros增强的AH109A细胞侵袭的抑制作用有关。
Resveratrol, present in grapes and other plants, is a polyphenolic compound with strong antioxidative activity. In our previous studies. we found that reactive oxygen species (ROS) accelerated the invasive capacity of a rat ascites hepatoma cell line of AH109A in culture and that resveratrol and resveratrol-loaded rat sera suppressed the ROS-potentiated invasion of the hepatoma cells. To study mechanisms by which resveratrol and its in vivo metabolite(s) suppress the invasion, we estimated intracellular peroxide level and expression of hepatocyte growth factor (HGF), a known cell motility factor, in AH109A cells. Exogenously added ROS promoted the intracellular peroxide level and the expression of HGF. Resveratrol and resveratrol-loaded rat sera canceled the rise in the peroxide level and HGF expression in ROS-stimulated tumor cells. These results suggest an involvement of the antioxidative property of resveratrol and sera from rats orally given resveratrol in their suppressive effects on ROS-potentiated invasion of AH109A cells.