Occurrence of tetrodotoxin as the major toxin in a xanthid crab Atergatis floridus.
Occurrence of tetrodotoxin as the major toxin in a xanthid crab Atergatis floridus.
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DOI：
10.2331/suisan.49.1887
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发表时间：
1983
影响因子：
0.4
通讯作者：
T. Noguchi;A. Uzu;Kinue Koyama;J. Maruyama;Y. Nagashima;K. Hashimoto
中科院分区：
文献类型：
--
作者：
T. Noguchi;A. Uzu;Kinue Koyama;J. Maruyama;Y. Nagashima;K. Hashimoto
采用活性炭处理、Amberlite IRC-50、Bio-Gel P-2和Bio-Rex 70柱层析等方法，对产自三浦半岛的黄蟹Atergatisfloridus毒素进行了纯化。在Bio-Rex 70柱层析中，相当于总毒性约90%的主要毒素用0-0.03 N乙酸洗脱，而次要毒素用0.03-1 N乙酸洗脱。主毒素在薄层色谱和柱层析中均为单一斑点，Rf和Rm值与河豚毒素对照品一致。此外，主要毒素在NMR谱中与河豚毒素一致。根据这些结果，主要毒素被鉴定为河豚毒素。另一方面，微量毒素被证明由蛤蚌毒素和一些未鉴定的有毒成分组成。
The toxins of a xanthid crab Atergatis floridus from Miura Peninsula were purified by a method which consisted of activated charcoal treatment, and chromatography on Amberlite IRC-50, Bio-Gel P-2 and Bio-Rex 70 columns. In the Bio-Rex 70 column chromatography, the major toxin equivalent to about 90% of the total toxicity was eluted with 0-0.03 N acetic acid, whereas the minor toxin, with 0.03-1 N acetic acid. The major toxin showed a single spot in TLC and electrophoreis, whose Rf and Rm values coincided well with those of tetrodotoxin run for reference. Furthermore, the major toxin agreed well with tetrodotoxin in NMR spectrum. From these results, the major toxin was identified as tetrodotoxin. On the other hand, the minor toxin was demonstrated to consist of saxitoxin and some unidentified toxic components.