Site-directed antibody immobilization on gold substrate for surface plasmon resonance sensors
Site-directed antibody immobilization on gold substrate for surface plasmon resonance sensors
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DOI：
10.1016/j.snb.2005.03.018
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发表时间：
2006-01-17
影响因子：
8.4
通讯作者：
Suri, CR
中科院分区：
文献类型：
--
作者：
Schmid, AH;Stanca, SE;Suri, CR
发展了一种用于表面等离子体共振(SPR)的简单方法，用于在金基质上定点固定抗体。用蛋白A通过同双功能交联剂二硫代双琥珀酰亚胺丙酸酯(DSP)对玻片上新制备的金表面进行修饰，以获得均匀、稳定和空间可及的抗体涂层。考察了蛋白质浓度、缓冲液pH、缓冲液类型和反应时间对蛋白A结合抗体密度的影响。该方案还被用于石英晶体微天平(QCM)镀金基片的改性。修饰后的金表面稳定数周，重现性良好。目前的制造技术可以应用于构建其他免疫传感器或生物芯片。(C)2005年，爱思唯尔出版。
A simple method for the site-directed immobilization of antibodies on gold substrates has been developed for surface plasmon resonance (SPR) applications. A freshly prepared gold surface on a glass slide was modified with Protein A via homobifunctional cross linker dithiobissuccinimide propionate (DSP) to achieve uniform, stable, and sterically accessible antibody coating. The density of the immobilized antibodies bound to Protein A was examined as a function of the protein concentration, buffer pH, buffer type and reaction time. The suggested scheme was also employed for the modification of gold-coated substrates for quartz crystal microbalance (QCM) applications. The modified gold surface was stable for several weeks and the reproducibility was satisfactory. The current fabrication technique could be applied to construct other immunosensors or biochips. (c) 2005 Published by Elsevier B.V.