Rapid separation and sensitive determination of banned aromatic amines with plastic microchip electrophoresis.
Rapid separation and sensitive determination of banned aromatic amines with plastic microchip electrophoresis.
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DOI：
10.1016/j.jhazmat.2013.01.010
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发表时间：
2013-03
影响因子：
13.6
通讯作者：
Ruina Li;Lili Wang;Xiaotong Gao;Gangfeng Du;H. Zhai;Xiayan Wang;G. Guo;Q. Pu
中科院分区：
文献类型：
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作者：
Ruina Li;Lili Wang;Xiaotong Gao;Gangfeng Du;H. Zhai;Xiayan Wang;G. Guo;Q. Pu
环境样品和生活必需品中痕量芳香胺的快速分析已经引起了人们的广泛关注，因为其中一些芳香胺具有强烈的毒性和致癌性。本研究探索了利用一次性塑料芯片结合低成本激光诱导荧光检测器对5种禁用芳香胺进行快速、高效的电泳法分离和灵敏检测的方法。系统考察了运行缓冲液及其添加剂的作用效果。在选定的条件下，使用含2%(w/v)羟丙基纤维素的10 mmol/L硼酸盐缓冲液，可在90s内基线分离5种荧光素异硫氰酸酯标记的芳香胺。不同分析物的峰面积与浓度的线性范围分别为40或120nmoL/L，检出限为1~3nmoL/L，理论板数为6.8~8.5×10~5/m。方法重现性好，峰面积和迁移时间的相对标准偏差(n=5)分别不超过4.6%和0.9%。所建立的方法用于实际废水和纺织样品中禁用芳香胺的定量测定，加标回收率为85~110%。
Rapid analysis of trace amount of aromatic amines in environmental samples and daily necessities has attracted considerable attentions because some of them are strongly toxic and carcinogenic. In this study, fast and efficient electrophoretic separation and sensitive determination of 5 banned aromatic amines were explored for practical analysis using disposable plastic microchips combined with a low-cost laser-induced fluorescence detector. The effect of running buffer and its additive was systematically investigated. Under the selected condition, 5 fluorescein isothiocyanate labeled aromatic amines could be baseline separated within 90s by using a 10mmol/L borate buffer containing 2% (w/v) hydroxypropyl cellulose. Calibration curves of peak areas vs. concentrations were linear up to 40 or 120μmol/L for different analytes and limits of detection were in a range of 1–3nmol/L. Theoretical plate numbers of 6.8–8.5×105/m were readily achieved. The method exhibited good repeatability, relative standard deviations (n=5) of peak areas and migration times were no more than 4.6% and 0.9%, respectively. The established method was successfully applied in the quantitative analysis of these banned aromatic amines in real samples of waste water and textile, recoveries of added standards were 85–110%.