Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp isolate ANT-6
Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp isolate ANT-6
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DOI：
10.1016/s0032-9592(03)00037-2
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发表时间：
2003-05-30
影响因子：
4.4
通讯作者：
Osman, G
中科院分区：
文献类型：
--
作者：
Burhan, A;Nisa, U;Osman, G
从土壤样品中分离到一株产高温碱性α-淀粉酶的芽孢杆菌ANT-6。酶的合成发生在25至45摄氏度之间的温度，最佳温度为37摄氏度。在pH范围7和11内淀粉酶合成略有变化，最佳pH为9。产酶和生长的最适温度相同。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析该酶，发现在淀粉凝胶中检测到一条显示淀粉分解活性的条带。该酶的相对分子质量为94500 Da，最适pH为10.5，最适温度为80 ℃。部分纯化的酶在pH（9.5-13）的碱性范围内具有高活性，并且在pH 10.5时，直到100 ℃仍保持85.5%的初始活性。在5 mM CaCl 2（110%）和3 mM PMSF（103%）的存在下，酶活性得到增强，并且通过Zn、Na、Na-sulfide、EDTA（10 mM）、尿素（8 M）和SDS（0.1%）在5 mM下分别获得36.9%、21.5%、22.2%、4.90%、86%和10.27%的抑制。该酶在高碱性pH下24 h稳定性为55%。因此，我们的研究结果表明，该酶是碱性，热稳定，热电堆和螯合剂的阻力。ANT-6淀粉酶可适用于淀粉液化、洗涤剂和纺织工业以及其它工业应用。(C)2003爱思唯尔科技有限公司版权所有。
A thermostable alkaline a-amylase producing Bacillus sp. ANT-6 was isolated from soil samples. Enzyme synthesis occurred at temperatures between 25 and 45 degreesC with an optimum of 37 degreesC. There was a slight variation in amylase synthesis within the pH range 7 and 11 with an optimum pH of 9. The optimum temperatures for amylase production and growth were the same. Analyses of the enzyme by sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed a single band, which show amylolytic activity, detected in starch gel. The relative molecular mass of the partial purified enzyme was estimated to be 94 500 Da. The enzyme showed optimum activity at pH 10.5 and 80 degreesC. The partial purified enzyme was highly active in the alkaline range of pH (9.5-13), and it was completely active up to 100 degreesC retaining 85.5% initial activity at pH 10.5. Enzyme activity was enhanced in the presence of 5 mM CaCl2 (110%) and 3 mM PMSF (103%), and inhibition with 5 mM by Zn, Na, Na-sulphide, EDTA (10 mM), Urea (8 M) and SDS (0.1%) was obtained 36.9, 21.5, 22.2, 4.90, 86% and 10.27, respectively. The enzyme was stable (55%) at high alkaline pH for 24 h. So our result showed that the enzyme was both alkaline, thermostable, thermopile and chelator resistant. The ANT-6 amylase enzyme may be suitable in liquefaction of starch, in detergent and textile industries and in other industrial applications. (C) 2003 Elsevier Science Ltd. All rights reserved.