Role of adenosine triphosphate, active mitochondria, and microtubules in the acquisition of developmental competence of parthenogenetically activated pig oocytes
Role of adenosine triphosphate, active mitochondria, and microtubules in the acquisition of developmental competence of parthenogenetically activated pig oocytes
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DOI：
10.1095/biolreprod.104.038141
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发表时间：
2005-05-01
影响因子：
3.6
通讯作者：
Gandolfi, F
中科院分区：
文献类型：
--
作者：
Brevini, TAL;Vassena, R;Gandolfi, F
这项工作的目的是确定猪卵母细胞获得细胞质成熟和胚胎发育能力的调节机制。成熟培养基中是否存在猪卵泡液（pff；25%或0%）被用作实现伴随或不伴随细胞质成熟的完全核成熟的手段。在体外成熟 (IVM) 过程中的不同时间分析了 ATP 含量、活性线粒体重定位和微管分布。虽然两组之间的核成熟没有差异，但 25% pff 组的孤雌胚胎发育 (41.5%) 显着高于 0% pff 组 (19.0%)，囊胚的卵裂球数量显着更高 (分别为 76.1 +/- 6.3 和 47.2 +/- 6.5)。 IVM 期间卵母细胞 ATP 含量显着增加，但在成熟结束时，高能力和低能力卵母细胞之间没有观察到显着差异。仅在高能力卵母细胞组中观察到在 lVM 过程中线粒体广泛重新定位到内部细胞质，并形成发育良好的细胞质微管网。然而，在高能力组和低能力组之间没有观察到与减数分裂纺锤体相关的微管形成存在差异。我们得出的结论是，发育能力低下与微管细胞质网络的缺乏有关，这阻碍了线粒体的正确重新定位，并且可能反映了卵质的更普遍的改变。这可以独立于完成染色体分离所需的微管机制的形成，并且不影响总的 ATP 含量。
The purpose of this work was to determine the mechanisms regulating the acquisition of cytoplasmic maturation and embryonic developmental competence in pig oocytes. The presence or the absence of porcine follicular fluid (pff; 25% or 0%) in the maturation medium was used as a means to achieve complete nuclear maturation accompanied or not accompanied by cytoplasmic maturation. ATP content, active mitochondria relocation, and microtubule distribution were analyzed at different times during in vitro maturation (IVM). While nuclear maturation did not differ among the two groups, parthenogenetic embryonic development was significantly higher (41.5%) in the 25% pff group than in the 0% pff group (19.0%) with blastocysts that had a significantly higher number of blastomeres (76.1 +/- 6.3, and 47.2 +/- 6.5, respectively). Oocyte ATP content increased significantly during IVM, but at the end of maturation no significant differences were observed between high- and low competence oocytes. An extensive relocation of mitochondria to the inner cytoplasm during lVM together with the formation of a well-developed mesh of cytoplasmic microtubules was observed only in the high-competence oocyte group. However, no differences in the formation of microtubules associated with the meiotic spindles were observed between high- and low-competence groups. We conclude that low developmental competence is associated with the lack of a microtubule cytoplasmic network, which prevents correct relocation of mitochondria and is likely to reflect a more generally altered compartmentalization of the ooplasm. This can be independent from the formation of the microtubule machinery required for the completion of chromosome disjunctions and does not affect the overall ATP content.