Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein
Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein
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DOI：
10.1021/bp000057p
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发表时间：
2000-07-01
影响因子：
2.9
通讯作者：
Wood, HA
中科院分区：
文献类型：
--
作者：
Joshi, L;Davis, TR;Wood, HA
研究了用杆状病毒表达载体系统在昆虫细胞中制备哺乳动物型人蛋白复合n链糖基化所需的条件。在不同的杆状病毒种类、昆虫细胞系和细胞培养基中观察到人胎盘分泌碱性磷酸酶（SEAP） n -链糖基化的显著变化。用重组加州自签名核多角体病毒（AcMNPV）在无血清培养基中培养的ni毛癣（Tn-4h）细胞中产生SEAP，结构分析表明
The conditions required for mammalian-type complex N-linked glycosylation of human proteins produced in insect cells with the baculovirus expression vector system were investigated. Marked alterations to N-linked glycosylation of human placental secreted alkaline phosphatase (SEAP) were observed with different baculovirus species, insect cell lines, and cell culture media. When a recombinant Autographa californica nucleopolyhedrovirus (AcMNPV) was used to produce SEAP in Trichoplusia ni (Tn-4h) cells cultured in serum-free medium, structural analyses indicated