The novel gene apnoia regulates Drosophila tracheal tube size.
The novel gene apnoia regulates Drosophila tracheal tube size.
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新基因 apnoia 调节果蝇气管的大小。
DOI：
10.1002/dvdy.29
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Jiang,Lan
中科院分区：
文献类型：
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作者：
Scholl,Aaron;O'Brien,MichaelJ;Chandran,RachanaR;Jiang,Lan
不同的管大小对人体管状器官（如肺、血管系统和肾脏）的功能至关重要。异常的试管大小会导致严重的人类疾病，包括多囊肾病。果蝇气管提供了一个首要的遗传系统来研究调节气管大小的基本机制。结果我们描述了一个新的基因，呼吸障碍，在管大小调节中的功能。apenenii编码一个顶膜蛋白Apnoia (Apn)，具有三个螺旋跨膜结构域。功能缺失突变体在幼虫气管中表现出短管和充气性缺陷，而在胚胎气管中没有明显缺陷。相反，气管apnin的过表达会导致气管明显过伸。我们分析了这些长管的根尖管腔基质和细胞极性。有趣的是，我们观察到细胞极性的正常建立，而所有管腔基质成分都显着减少。此外，我们观察到一些基质成分定位于细胞质囊泡中，这表明在apnor表达气管中存在分泌缺陷。结论本研究结果提示，白内障可能直接或间接参与了肾小泡运输，从而调节肾小泡的大小。
BackgroundDistinct tube size is critical for the function of human tubular organs such as the lung, vascular system, and kidney. Aberrant tube sizes can lead to devastating human illnesses, including polycystic kidney disease. TheDrosophilatrachea provides a premier genetic system to investigate the fundamental mechanisms that regulate tube size.ResultsHere we describe the function of a novel gene,apnoia, in tube‐size regulation.apnencodes an apical membrane protein, Apnoia (Apn), with three helical transmembrane domains. Loss‐of‐functionapnmutants show shorter‐tube and air‐filling defects in larval trachea, whereas there are no obvious defects in embryonic trachea. Conversely, overexpression ofapnin trachea leads to significant tube over‐elongation. We analyzed apical luminal matrix and cell polarity in these longer tubes. It is interesting to note that we observed normal establishment of cell polarity, whereas all luminal matrix components are significantly reduced. In addition, we observed that some matrix components are localized in cytoplasmic vesicles, suggesting secretion defects inapnoverexpressing trachea.ConclusionTaken together, these results strongly suggest the possibility thatapnis directly or indirectly involved in vesicular trafficking to regulate tube size.