Efficacy of two entomopathogenic nematode species as potential biocontrol agents against the rosaceae longhorned beetle, Osphranteria coerulescens, under laboratory conditions
Efficacy of two entomopathogenic nematode species as potential biocontrol agents against the rosaceae longhorned beetle, Osphranteria coerulescens, under laboratory conditions
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实验室条件下两种昆虫病原线虫作为潜在生物防治剂对抗蔷薇科长角甲虫 Osphranteria coerulescens 的功效
DOI：
10.1163/15685411-00002802
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
M. Rezapanah
中科院分区：
文献类型：
--
作者：
S. Sharifi;Javad Karimi;M. Hosseini;M. Rezapanah
蔷薇科天牛是伊朗寒冷地区重要的果树害虫，危害蔷薇科所有果树。在实验室条件下，测定了两种昆虫病原线虫（EPN）-异小杆线虫（Heterorhabditis bacteriophora）和果荚斯氏线虫（Steinernema carpocapsae）对该害虫末龄幼虫的潜在防治效果。在平板试验中，幼虫对两种EPN都敏感，但对S.果荚螨的死亡率为65.0-97.5%，而黑果螨的死亡率为65.0-97.5%。细菌门（42.5-87.8%）。两种线虫均能在O. coerulescens幼虫的繁殖率最高，而H. bacteriophora比S. carpocapsae的渗透率; carpocapsae比H.细菌门。在琼脂平板迁移试验中，S. carpocapsae表现出可忽略不计的吸引力的害虫或大蜡螟线索。然而，H.细菌被强烈吸引到含有幼虫的培养皿的扇区。在杏树枝条试验中，两种EPN均从O.在枝梢处的幼虫，侵入幼虫廊道，定位并杀死了O. coerulescens在它们的自然栖息地深处的分支机构。我们的研究结果提供了第一次深入了解的生物防治效果的EPN对O。coerulescens幼虫，并强调其控制这种害虫的潜力。需要进行田间试验，以评估其在发现蔷薇科天牛幼虫的环境条件下的潜力。
Rosaceae longhorned beetle, Osphranteria coerulescens, is an important pest of fruit trees that attacks all fruit trees belonging to the family Rosaceae in cold regions of Iran. The potential efficacy of two species of entomopathogenic nematodes (EPN), Heterorhabditis bacteriophora and Steinernema carpocapsae, against last instar larvae of this pest was tested under Laboratory conditions. In plate assays, the larvae were susceptible to both EPN species but were more susceptible to S. carpocapsae (65.0-97.5% mortality) than H. bacteriophora (42.5-87.8%). Both nematode species were able to penetrate and reproduce within O. coerulescens larvae, but reproduction rate for H. bacteriophora was higher than those of S. carpocapsae; however, the penetration rate for S. carpocapsae was greater than H. bacteriophora. In a migration test on agar plate, S. carpocapsae showed negligible attraction to the pest or to Galleria mellonella cues. However, H. bacteriophora was strongly attracted to the sector of Petri dishes containing larvae. In a test using apricot tree branches, both species of EPN passed from mass frass of O. coerulescens larvae in the tip of the branches, penetrated into the larval galleries, and located and killed the larvae of O. coerulescens in their natural habitat deep inside the branches. Our findings provide the first insight into the biocontrol efficacy of EPN against O. coerulescens larvae, and highlight their potential for the control of this pest. Field experiments are needed to evaluate their potential under the environmental conditions in which rosaceae longhorned beetle larvae are found.