Close genetic and physical linkage between the murine haemopoietic growth factor genes GM‐CSF and Multi‐CSF (IL3).
Close genetic and physical linkage between the murine haemopoietic growth factor genes GM‐CSF and Multi‐CSF (IL3).
复制标题
小鼠造血生长因子基因 GM-CSF 和 Multi-CSF (IL3) 之间存在密切的遗传和物理联系。
DOI：
10.1002/j.1460-2075.1987.tb04799.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
N. Gough
中科院分区：
文献类型：
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作者：
D. Barlow;M. Bucan;H. Lehrach;B. Hogan;N. Gough
两种小鼠造血生长因子，粒细胞-巨噬细胞集落刺激因子（GM - CSF）和多- CSF（白细胞介素3）刺激一组重叠的造血祖细胞的增殖和分化，并在T淋巴细胞激活后协调产生。在这里，我们报告了编码这两个因素的基因的染色体位置。最初，通过对小鼠/中国仓鼠体细胞杂交的分析，将这两个基因分配到第11染色体上。利用种间（小家鼠与小家鼠）回交的遗传分析证实了这一分配，证明GM - CSF和Multi - CSF基因都与SPARC基因有遗传联系，SPARC基因被独立分配到11号染色体B1亚带。脉冲场凝胶电泳的物理距离分析进一步表明，两个脑脊液基因之间的距离在230 kb以内。然而，通过脉冲场凝胶分析检查包含所有三个基因座的亚染色体区域显示，SPARC距离包含两个CSF基因的区域至少400 - 500 kb。
The two murine haemopoietic growth factors, granulocyte‐macrophage colony stimulating factor (GM‐CSF) and Multi‐CSF (interleukin 3) stimulate the proliferation and differentiation of an overlapping set of haemopoietic progenitor cells and are produced coordinately following activation of T lymphocytes. Here we report the chromosomal location of the genes encoding these two factors. Initially both genes were assigned to chromosome 11 by analysis of mouse/Chinese hamster somatic cell hybrids. Genetic analysis using an interspecies (Mus musculus X Mus spretus) back‐cross confirmed this assignment by demonstrating that both the GM‐CSF and Multi‐CSF genes are genetically linked to the SPARC gene, which had been independently assigned to sub‐band B1 of chromosome 11. Analysis of physical distances by pulsed field gel electrophoresis demonstrated further that the two CSF genes lie within 230 kb of each other. However examination of the subchromosomal region containing all three loci by pulsed field gel analysis showed that SPARC is at least 400‐500 kb distant from the region containing the two CSF genes.