The volatile organic compounds ofFloccularia luteovirensmodulate plant growth and metabolism inArabidopsis thaliana
The volatile organic compounds ofFloccularia luteovirensmodulate plant growth and metabolism inArabidopsis thaliana
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黄绿絮藻挥发性有机化合物调节拟南芥植物生长和代谢
DOI：
10.1007/s11104-020-04709-8
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发表时间：
2020
期刊：
影响因子：
4.9
通讯作者：
Xu Jin
中科院分区：
文献类型：
--
作者：
Sun Liangliang;Cao Ming;Liu Fei;Wang Yibo;Wan Jinpeng;Wang Ruling;Zhou Huakun;Wang Wenying;Xu Jin
目的土壤微生物产生的挥发性有机化合物(VOCs)对植物的生长发育起着重要的调节作用。黄腐菌是一种食用菌，对青藏高原高寒草甸植物的生长具有重要的促进作用。本研究旨在阐明蘑菇真菌VOC介导植物生长发育的生理和分子机制。利用1/2MS培养基组培苗，综合生理学、遗传学、转录组和代谢组分析，研究黄萎病对拟南芥根系发育和幼苗生长的影响。黄绿假单胞菌VOCs通过抑制生长素外流载体PIN2(PIN2)蛋白的丰度而加剧生长素的积累，从而减少了初生根的生长，而增加了侧根的数量。那就是树苗。除调控根系构型外，F.黄酮类挥发性有机化合物显著促进了地上部分的生长。转录组和代谢组分析进一步支持了这一观点。黄绿假单胞菌VOCs通过诱导碳氮代谢和抗氧化防御来调节植物的生长发育，同时抑制多种次生代谢途径和氨基酸分解代谢途径。黄绿假单胞菌VOCs通过生长素途径诱导根系构型改变，调节植物体内的代谢，从而促进植物生长。
AimsThe volatile organic compounds (VOCs) produced by soil microbes modulated plant growth and development.Floccularia luteovirens, an edible mushroom, is beneficial to the growth of alpine meadow plants on the Qinghai-Tibet Plateau. We aimed to elucidate the physiological and molecular mechanisms underlying the mushroom fungal VOC-mediated plant growth and development.MethodsHere, we investigated the effects of VOCs produced byF. luteovirenson the root system development and seedling growth by integrating physiology, genetics, transcriptome and metabolome analysis using 1/2 MS medium-grownArabidopsis thalianaseedlings.ResultsTreatment withF. luteovirensVOCs reduce primary root growth by aggravating auxin accumulation through the repression of the abundance of auxin efflux carrier PIN-FORMED 2 (PIN2) protein, whereas it increases the lateral root number ofA. thalianaseedlings. In addition to modulating root system architecture, treatment withF. luteovirensVOCs markedly increased aboveground growth. The transcriptome and metabolome analyses further supported the notion thatF. luteovirensVOCs modulate plant growth and development through the induction of carbon/nitrogen metabolism and antioxidant defense while repressing several secondary metabolism and amino acid catabolism pathways.ConclusionsThese results suggested that application ofF. luteovirensVOCs promote growth by inducing changes in root system architecture through auxin pathway and regulating metabolism in plants.