Interaction between Bacteriophage DMS3 and Host CRISPR Region Inhibits Group Behaviors of Pseudomonas aeruginosa
Interaction between Bacteriophage DMS3 and Host CRISPR Region Inhibits Group Behaviors of Pseudomonas aeruginosa
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DOI：
10.1128/jb.00797-08
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
O'Toole, George A.
中科院分区：
文献类型：
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作者：
Zegans, Michael E.;Wagner, Jeffrey C.;O'Toole, George A.
噬菌体感染对细菌生物学有着深远的影响。簇状规则间隔短回文重复序列（CRISPR）和cas （CRISPR相关）基因存在于大多数古细菌和许多细菌中，据报道它们在抵抗噬菌体感染中发挥作用。我们观察到铜绿假单胞菌PA14与噬菌体DMS3的溶原性感染抑制了生物膜的形成和群体运动，这两个重要的细菌群体行为。这种抑制需要宿主中的CRISPR区域。突变或删除6个cas基因中的5个以及该区域2个crispr中的1个，可以恢复DMS3溶原菌株的生物膜形成和群集。我们的观察结果表明，CRISPR区域在修改铜绿假单胞菌溶原作用中的作用。
Bacteriophage infection has profound effects on bacterial biology. Clustered regular interspaced short palindromic repeats (CRISPRs) and cas (CRISPR- associated) genes are found in most archaea and many bacteria and have been reported to play a role in resistance to bacteriophage infection. We observed that lysogenic infection of Pseudomonas aeruginosa PA14 with bacteriophage DMS3 inhibits biofilm formation and swarming motility, both important bacterial group behaviors. This inhibition requires the CRISPR region in the host. Mutation or deletion of five of the six cas genes and one of the two CRISPRs in this region restored biofilm formation and swarming to DMS3 lysogenized strains. Our observations suggest a role for CRISPR regions in modifying the effects of lysogeny on P. aeruginosa.