Immunoglobulin isotype expression II. Frequency analysis in mitogen‐reactive B cells
Immunoglobulin isotype expression II. Frequency analysis in mitogen‐reactive B cells
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丝裂原反应性 B 细胞中的免疫球蛋白同种型表达频率分析
DOI：
10.1002/eji.1830111012
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发表时间：
1981
影响因子：
5.4
通讯作者：
H. Hooijkaas
中科院分区：
文献类型：
--
作者：
R. Benner;A. Coutinho;A. Rijnbeek;A. van Oudenaren;H. Hooijkaas
在检测所有生长诱导型细胞的培养条件下，在体外测定了BALB/c小鼠细胞中脂多糖（LPS）反应性B细胞发育为分泌各种免疫球蛋白（IG）的克隆的频率。通过使用IgM、IgG 1、IgG 2a、IgG 2b、IgG 3或伊加的特异性显色抗血清，在蛋白A空斑试验中评估分泌IG的空斑形成细胞的不同IG类的分泌。在检测的所有淋巴器官（脾、骨髓、肠系膜淋巴结和胸导管）中，相当大比例的所有B细胞（5-20%）被LPS诱导产生IgM分泌细胞克隆。对LPS反应性细胞产生分泌其他IG同种型的后代的频率测定显示，平均而言，无论细胞来源如何，7%的IgM分泌克隆转化为IgG 1的合成，39%转化为IgG 2，41%转化为IgG 3，1%转化为伊加。在无胸腺裸鼠脾细胞中发现了大致相同的B细胞转换CH基因表达的频率。在多克隆激活的B细胞和体内“背景”IG分泌细胞中CH基因表达的克隆频率之间没有发现相关性，这表明B细胞中CH基因表达受到刺激质量和其他调节影响的影响。
The frequency of lipopolysaccharide (LPS)‐reactive B cells developing into clones that secrete the various immunoglobulin (Ig) classes has been determined in vitro, in cells from BALB/c mice, under culture conditions which detect all growth‐inducible cells. Secretion of the different Ig classes was assessed in the protein A plaque assay for Ig‐secreting, plaque‐forming cells by using developing antisera specific for either IgM, IgG1, IgG2a, IgG2b, IgG3 or IgA. In all lymphoid organs tested (spleen, bone marrow, mesenteric lymph nodes and thoracic duct), a considerable proportion of all B cells (5–20%) was induced by LPS to yield a clone of IgM‐secreting cells. Frequency determinations of LPS‐reactive cells giving rise to descendants secreting other Ig isotypes revealed that, on an average, and irrespective of the origin of the cells, 7% of all IgM‐secreting clones switched to the synthesis of IgG1, 39% to IgG2, 41% to IgG3 and 1% to IgA. Roughly the same frequencies of B cells switching CH gene expression were found among spleen cells of athymic nude mice. No correlation was found between the clonal frequencies of CH gene expression in polyclonally activated B cells and the in vivo “background” Ig‐secreting cells suggesting that the CH gene expression in B cells is influenced by the quality of stimulation and other regulating influences.