Anti-inflammatory activity of cinnamon water extract in vivo and in vitro LPS-induced models
Anti-inflammatory activity of cinnamon water extract in vivo and in vitro LPS-induced models
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DOI：
10.1186/1472-6882-12-237
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发表时间：
2012-11-28
影响因子：
--
通讯作者：
Kang, Hee
中科院分区：
文献类型：
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作者：
Hong, Joung-Woo;Yang, Ga-Eun;Kang, Hee
背景：肉桂皮是传统东方医学中最受欢迎的草药成分之一，具有多种药理活性，包括抗菌、抗病毒和抗癌特性。本研究的目的是探讨肉桂水提取物（CWE）对脂多糖（LPS）诱导的肿瘤坏死因子（TNF）-α的体内和体外抑制作用及其潜在的细胞内机制。方法：给小鼠口服CWE 6天，然后腹腔注射LPS。 LPS刺激后1小时测定TNF-α和白细胞介素(IL)-6的血清水平。分离来自注射了巯基乙酸盐的小鼠的腹膜巨噬细胞，并分析 LPS 刺激后的活力、细胞因子表达和信号分子。将CWE根据分子大小进一步分级，测定各组分的总多酚水平和生物活性。结果：小鼠口服CWE后，血清TNF-α和IL-6水平显着降低。体外 CWE 处理降低了 TNF-α 的 mRNA 表达。 CWE 阻断 LPS 诱导的 I kappa B α 降解以及 JNK、p38 和 ERK1/2 的激活。此外，CWE 基于大小的分级显示，在体外观察到的 CWE 抑制作用发生在总多酚含量最高的级分中。结论：CWE 治疗降低了血清中 LPS 诱导的 TNF-α。 CWE 对 TNF-α 基因的体外抑制可能是通过调节 I kappa B α 降解和 JNK、p38 和 ERK1/2 激活而发生的。我们的结果还表明，观察到的 CWE 抗炎作用可能源于多酚的存在。
Background: Cinnamon bark is one of the most popular herbal ingredients in traditional oriental medicine and possesses diverse pharmacological activities including anti-bacterial, anti-viral, and anti-cancer properties. The goal of this study is to investigate the in vivo and in vitro inhibitory effect of cinnamon water extract (CWE) on lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha and its underlying intracellular mechanisms.Methods: CWE was orally administrated to mice for 6 days prior to intraperitoneal injection of LPS. Serum levels of TNF-alpha and interleukin (IL)-6 were determined 1 hour after LPS stimulation. Peritoneal macrophages from thioglycollate-injected mice were isolated and assayed for viability, cytokine expression and signaling molecules upon LPS stimulation. CWE was further fractioned according to molecular size, and the levels of total polyphenols and biological activities of each fraction were measured.Results: The oral administration of CWE to mice significantly decreased the serum levels of TNF-alpha and IL-6. CWE treatment in vitro decreased the mRNA expression of TNF-alpha. CWE blocked the LPS-induced degradation of I kappa B alpha as well as the activation of JNK, p38 and ERK1/2. Furthermore, size-based fractionation of CWE showed that the observed inhibitory effect of CWE in vitro occurred in the fraction containing the highest level of total polyphenols.Conclusions: Treatment with CWE decreased LPS-induced TNF-alpha in serum. In vitro inhibition of TNF-alpha gene by CWE may occur via the modulation of I kappa B alpha degradation and JNK, p38, and ERK1/2 activation. Our results also indicate that the observed anti-inflammatory action of CWE may originate from the presence of polyphenols.