THE SACCHAROMYCES-CEREVISIAE YAK1-GENE ENCODES A PROTEIN-KINASE THAT IS INDUCED BY ARREST EARLY IN THE CELL-CYCLE
THE SACCHAROMYCES-CEREVISIAE YAK1-GENE ENCODES A PROTEIN-KINASE THAT IS INDUCED BY ARREST EARLY IN THE CELL-CYCLE
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DOI：
10.1128/mcb.11.8.4045
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发表时间：
1991-08-01
影响因子：
5.3
通讯作者：
BROACH, JR
中科院分区：
文献类型：
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作者：
GARRETT, S;MENOLD, MM;BROACH, JR
编码与已知蛋白激酶具有序列同源性的蛋白质的基因YAK1中的突变抑制缺乏环AMP依赖性蛋白激酶（A激酶）的突变体的细胞周期停滞表型。 也就是说，Yak1蛋白的损失专门补偿A激酶的损失。 在这里，我们表明由YAK1编码的蛋白具有蛋白激酶活性。 Yak1激酶活性在指数生长期间较低，但在S期完成之前通过细胞停滞诱导至少50倍。 在细胞周期的后期阶段，通过阻滞未观察到诱导。 根据阻滞方案，诱导可以通过增加Yak1蛋白水平或通过增加Yak1比活性来发生。 最后，Yak 1蛋白水平的增加会导致A激酶活性减弱的细胞生长停滞。 这些结果表明，Yak1的行为在一个途径平行的A激酶负调控细胞增殖。
Null mutations in the gene YAK1, which encodes a protein with sequence homology to known protein kinases, suppress the cell cycle arrest phenotype of mutants lacking the cyclic AMP-dependent protein kinase (A kinase). That is, loss of the Yak1 protein specifically compensates for loss of the A kinase. Here, we show that the protein encoded by YAK1 has protein kinase activity. Yak1 kinase activity is low during exponential growth but is induced at least 50-fold by arrest of cells prior to the completion of S phase. Induction is not observed by arrest at stages later in the cell cycle. Depending on the arrest regimen, induction can occur either by an increase in Yak1 protein levels or by an increase in Yak1 specific activity. Finally, an increase in Yak1 protein levels causes growth arrest of cells with attenuated A kinase activity. These results suggest that Yak1 acts in a pathway parallel to that of the A kinase to negatively regulate cell proliferation.