A Hydrogel-Based Hybrid Theranostic Contact Lens for Fungal Keratitis
A Hydrogel-Based Hybrid Theranostic Contact Lens for Fungal Keratitis
复制标题
基于水凝胶的混合治疗隐形眼镜治疗真菌性角膜炎
DOI：
10.1021/acsnano.6b00601
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发表时间：
2016-07-01
期刊：
影响因子：
17.1
通讯作者：
Jiang, Gang-Biao
中科院分区：
文献类型：
--
作者：
Huang, Jian-Fei;Zhong, Jing;Jiang, Gang-Biao
真菌性角膜炎是一种严重的眼部疾病，是眼部发病和失明的主要原因之一，但它往往被忽视，特别是在发展中国家。传统的治疗方法如滴眼液由于生物利用度差，疗效非常有限，而眼内注射可能会引起严重的副作用。在此，我们设计并制造了一种混合水凝胶基接触透镜，其包含季铵化壳聚糖（HTCC）、银纳米颗粒和氧化石墨烯（GO），具有抗细菌和抗真菌功能的组合。该水凝胶通过GO和HTCC之间的静电相互作用交联，从而产生强大的机械性能。伏立康唑（Vor）是一种抗真菌药物，可以负载到GO上，GO保留药物并促进其从水凝胶接触镜中持续释放。鉴于甘氨酰三甲基氯化铵和银纳米颗粒，接触镜也表现出良好的抗菌功能。体外和体内实验结果表明，负载Vor的角膜接触镜在体外具有良好的抗真菌活性，并且可以显著增强对真菌感染小鼠模型的治疗效果。结果表明，这种基于水凝胶接触镜的药物递送系统可能是一种有前途的治疗方法，用于快速有效地治疗真菌性角膜炎。
Fungal keratitis, a severe ocular disease, is one of the leading causes of ocular morbidity and blindness, yet it is often neglected, especially in developing countries. Therapeutic efficacy of traditional treatment such as eye drops is very limited due to poor bioavailability, whereas intraocular injection might cause serious side effects. Herein, we designed and fabricated a hybrid hydrogel-based contact lens which comprises quaternized chitosan (HTCC), silver nanoparticles, and graphene oxide (GO) with a combination of antibacterial and antifungal functions. The hydrogel is cross-linked through electrostatic interactions between GO and HTCC, resulting in strong mechanical properties. Voriconazole (Vor), an antifungal drug, can be loaded onto GO which retains the drug and promotes its sustained release from the hydrogel-based contact lenses. The contact lenses also exhibited good antimicrobial functions in view of glycidyltrimethylammonium chloride and silver nanoparticles. The results from in vitro and in vivo experiments demonstrate that contact lenses loaded with Vor have excellent efficacy in antifungal activity in vitro and could significantly enhance the therapeutic effects on a fungus-infected mouse model. The results indicate that this hydrogel contact lenses-based drug delivery system might be a promising therapeutic approach for a rapid and effective treatment of fungal keratitis.