Transcriptome analyses of differential gene expression in the bursa of Fabricius between Silky Fowl and White Leghorn.
Transcriptome analyses of differential gene expression in the bursa of Fabricius between Silky Fowl and White Leghorn.
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乌骨鸡和白来航法氏囊差异基因表达的转录组分析
DOI：
10.1038/srep45959
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发表时间：
2017-04-13
影响因子：
4.6
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Han D;Zhang Y;Chen J;Hua G;Li J;Deng X;Deng X
丝鸡色素沉着导致免疫细胞发育异常。然而，黑素细胞如何调节法氏囊（BF）中的b细胞增殖尚不清楚。为了解决这一难题，我们收集了三周龄的SF和White Leghorn （WL）母鸡的BFs进行RNA测序。顺丰区的BF发育相对弱于WL。转录组分析在SF的BF中鉴定出4848个差异表达基因，326个长链非编码rna （lncrna）和67个microrna。与黑色素形成相关的基因在SF中显著高于WL，而与细胞因子-细胞因子受体相互作用和JAK-STAT信号通路相关的基因在SF中显著低于WL。关键的生物学过程，如受体活性、细胞通讯和细胞对刺激的反应，都聚集在SF中。预测的lncRNAs靶基因主要与细胞增殖通路相关，如JAK-STAT、WNT、MAPK和Notch信号通路。除上述途径外，靶microRNA基因还与代谢、黑色素生成、自噬、nod样和toll样受体信号通路相关。预计lncrna和microrna可调控JAK2、STAT3和IL-15基因。因此，SF BF中的b细胞发育可能受到非编码rna的调控和影响。
Hyperpigmentation in Silky Fowl (SF) results in aberrant immune cell development. However, how melanocytes regulate B-cell proliferation in the bursa of Fabricius (BF) is unclear. To resolve this conundrum, we collected BFs from three-week-old SF and White Leghorn (WL) female chickens for RNA sequencing. The BF development was relatively weaker in SF than in WL. The transcriptome analyses identified 4848 differentially expressed genes, 326 long noncoding RNAs (lncRNAs), and 67 microRNAs in the BF of SF. The genes associated with melanogenesis was significantly higher, but that of the genes associated with the cytokine-cytokine receptor interactions and JAK-STAT signalling pathway was significantly lower in SF than in WL. Crucial biological processes, such as the receptor activity, cell communication, and cellular responses to stimuli, were clustered in SF. The predicted target lncRNAs genes were mainly associated with cell proliferation pathways such as JAK-STAT, WNT, MAPK, and Notch signalling pathways. Except for the above pathways, the target microRNA genes were related to the metabolism, melanogenesis, autophagy, and NOD-like and Toll-like receptor signalling pathways. The lncRNAs and microRNAs were predicted to regulate the JAK2, STAT3, and IL-15 genes. Thus, B-cell development in the BF of SF might be regulated and affected by noncoding RNAs.