CXCL5 overexpression is associated with late stage gastric cancer
CXCL5 overexpression is associated with late stage gastric cancer
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DOI：
10.1007/s00432-007-0225-x
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发表时间：
2007-11-01
影响因子：
3.6
通讯作者：
Song, Si Young
中科院分区：
文献类型：
--
作者：
Park, Jeong Youp;Park, Kyung Hwa;Song, Si Young
目的趋化因子在多种肿瘤的发生、发展过程中起着重要作用。我们的cDNA阵列数据表明，趋化因子CXCL 5在胃癌中上调。在这里，我们分析了CXCL 5蛋白在胃癌中的表达，并探讨了CXCL 5上调的临床意义。当肿瘤组织染色比正常组织更强时，认为肿瘤组织中的免疫染色强度强;当肿瘤组织中的染色比正常组织中的染色弱时，认为强度为零;当两种组织中的染色相似时，认为强度弱。采用ELISA法和抗凝血因子VIII单克隆抗体检测血清CXCL 5水平和肿瘤组织微血管密度。CXCL 5表达与T分期无关。N分期与CXCL 5表达呈正相关。晚期（IIIB，IV）胃癌患者的血清CXCL 5水平高于良性疾病患者。CXCL 5强表达的肿瘤中微血管密度较高，但与CXCL 5的相关性不是线性的。多因素Logistic回归分析显示，与CXCL 5阴性或弱阳性表达相比，CXCL 5阳性表达是胃癌高N分期（N2、N3）的危险因素。这些结果表明CXCL 5在胃癌进展中的作用，特别是在淋巴结转移中。
Purpose Chemokines play multiple roles in the development and progression of many different tumors. Our cDNA array data suggested that chemokine CXCL5 was upregulated in gastric cancer. Here, we analyzed CXCL5 protein expression in gastric cancer and investigated the clinical implications of CXCL5 upregulation.Methods Immunostaining for CXCL5 was performed on gastric tissue microarrays of tissue specimens obtained by gastrectomy. The intensity of immunostaining in tumor tissue was considered strong when tumor tissue staining was more intense than in normal tissue; the intensity was null when staining was weaker in the tumor than in normal tissue; and the intensity was weak when staining was similar in both tissues. Serum CXCL5 levels and microvascular density in tumor tissue were measured by ELISA and monoclonal antibody to Factor VIII.Results Strong CXCL5 expression correlated with tumor stage. CXCL5 expression did not correlate with T stage. However, N stage positively correlated with CXCL5 expression. Serum CXCL5 levels in late stage (IIIB, IV) gastric cancer patients were higher than in patients with benign conditions. Microvascular density was higher in tumors with strong CXCL5 expression, but the correlation with CXCL5 was not linear. Multiple logistic regression analyses showed that, compared to no or weak expression, strong expression of CXCL5 was a significant risk factor for high N stage (N2, N3).Conclusion CXCL5 overexpression was associated with late stage gastric cancer and high N stage. These results suggest a role for CXCL5 in the progression of gastric cancer, specifically in lymph node metastasis.