CYTOSOLIC PH GRADIENTS ASSOCIATED WITH TIP GROWTH
CYTOSOLIC PH GRADIENTS ASSOCIATED WITH TIP GROWTH
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DOI：
10.1126/science.263.5152.1419
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发表时间：
1994-03-11
期刊：
影响因子：
56.9
通讯作者：
KROPF, DL
中科院分区：
文献类型：
--
作者：
GIBBON, BC;KROPF, DL
利用pH敏感微电极和比率成像技术研究了Pelvetia胚胎假根细胞中细胞质pH梯度的存在及其与极尖生长的关系。生长假根细胞产生的纵向pH梯度，其中顶端的细胞质是0.3至0.5个单位的酸性比细胞的基部的细胞质。用膜渗透性弱酸丙酸处理，消散了细胞溶质pH梯度并抑制生长。pH梯度的大小与尖端伸长的速率相关。的pH梯度空间上叠加的细胞溶质钙梯度，和抑制钙通量与镧治疗废除的pH梯度和抑制生长。
The presence of a cytosolic pH gradient and its relation to polar tip growth was investigated in rhizoid cells of Pelvetia embryos with the use of pH-sensitive microelectrodes and by ratio imaging. Growing rhizoid cells generated a longitudinal pH gradient in which the apical cytosol was 0.3 to 0.5 units more acidic than the cytosol at the base of the cell. Treatment with a membrane-permeant weak acid, propionic acid, dissipated the cytosolic pH gradient and inhibited growth. The magnitude of the pH gradient correlated well with the rate of tip elongation. The pH gradient spatially superimposed on the cytosolic calcium gradient, and inhibition of calcium fluxes by treatment with lanthanum abolished the pH gradient and inhibited growth.