GENE STRUCTURE OF HUMAN CARDIAC HORMONE PRECURSOR, PRONATRIODILATIN
GENE STRUCTURE OF HUMAN CARDIAC HORMONE PRECURSOR, PRONATRIODILATIN
复制标题
DOI：
10.1038/312654a0
复制
发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
CONDRA, JH
中科院分区：
文献类型：
--
作者：
NEMER, M;CHAMBERLAND, M;CONDRA, JH
心房心肌细胞含有典型的蛋白分泌细胞颗粒1，已知心房提取物含有强大的利钠利尿活性2，并具有平滑肌松弛活性3。已分离出多种活性心房肽，包括在大鼠4 - 9和人心房10中显示利钠、利尿和平滑肌松弛活性的相关肽家族;这些肽被命名为心房利钠因子（ANF）。另一种来自猪心房的无关肽11，心脏舒张素，被认为仅具有平滑肌松弛活性。其部分氨基酸序列与ANF序列无同源性。对ANF 12和ANF互补DNA克隆13 - 16的大形式（106个氨基酸）的序列分析表明，心舒张素和ANF肽是由共同的前体合成的。该前体还含有预期为分泌蛋白的信号肽序列17。我们现在描述这种新的激素前体的完整结构和人类基因序列，我们称之为前心房舒张素12。
Atrial cardiocytes contain granules typical of protein-secreting cells1, and atrial extracts are known to contain a powerful natriuretic and diuretic activity2and to possess smooth muscle relaxant activity3. A variety of active atrial peptides have been isolated, including a family of related peptides showing natriuretic, diuretic and smooth muscle relaxant activities in rat4–9and human atria10; these peptides were named atrial natriuretic factor (ANF). Another unrelated peptide from pig atria11, cardiodilatin, is thought to possess only smooth muscle relaxant activity. Its partial amino acid sequence shows no homology with ANF sequences. The sequence analysis of a large form (106 amino acids) of ANF12and of ANF complementary DNA clones13–16indicates that cardiodilatin and ANF peptides are synthesized from a common precursor. This precursor also contains a signal peptide sequence expected of a secretory protein17. We now describe the complete structure and sequence of the human gene for this novel hormone precursor that we call pronatriodilatin12.