Genotoxicity and Cytotoxicity of Multi-wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers
Genotoxicity and Cytotoxicity of Multi-wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers
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DOI：
10.1539/joh.l9150
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Fukushima, Shoji
中科院分区：
文献类型：
--
作者：
Asakura, Masumi;Sasaki, Toshiaki;Fukushima, Shoji
多壁碳纳米管对培养的中国仓鼠肺细胞的遗传毒性和细胞毒性与温石棉A纤维的比较目的：多壁碳纳米管(MWCNT)的潜在应用和工业化生产引起了人们对其对人类健康和环境安全的严重担忧。本研究旨在检测MWCNT和UICC温石棉A(温石棉)的体外细胞毒性和遗传毒性。方法：用不同浓度的MWCNT和温石棉处理培养的中国仓鼠肺(CHL/IU)细胞，用集落形成法和乳酸脱氢酶(LDH)法检测细胞毒性和染色体畸变率、微核率和hgprt致突变性。结果：MWCNT的体外细胞毒性与MWCNT混悬液的溶剂和超声作用时间有关。DMSO/培养基加超声作用3min，可得到分散性良好的培养基团聚集性最强的多壁碳纳米管。温石棉的细胞毒性比多壁碳纳米管更强。MWCNT的遗传毒性特征是形成多倍体，无结构染色体异常，双核和多核细胞数量增加，无微核诱导，hgprt阴性突变。温石棉表现出与MWCNT基本相同的遗传毒性，只是有轻微但显著的微核诱导。MWCNT和温石棉不完全内化于细胞内，定位于细胞质。结论：MWCNT和温石棉对中国仓鼠肺细胞具有细胞毒性和遗传毒性，但可能与DNA间接作用。结果表明，在胞质分裂过程中，这两种测试物质都会对生物过程产生物理干扰。(《眼镜报》健康版2010；52：155-166)
Genotoxicity and Cytotoxicity of Multi-wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers: Masumi ASAKURA, et al. Japan Bioassay Research Center, Japan Industrial Safety and Health Association Objectives: The potential applications and industrial production of multi-wall carbon nanotubes (MWCNT) have raised serious concerns about their safety for human health and the environment. The present study was designed to examine the in vitro cytotoxicity and genotoxicity of MWCNT and UICC chrysotile A (chrysotile). Methods: Cytotoxicity using both colony formation and lactate dehydrogenase (LDH) assays and genotoxicity including chromosome aberration, micronucleus induction and hgprt mutagenicity were examined by exposing cultured Chinese hamster lung (CHL/IU) cells to MWCNT or chrysotile at different concentrations. Results: The in vitro cytotoxicity of MWCNT depended on the solvent used for suspension of MWCNT and ultrasonication duration of the MWCNT suspension. A combination of DMSO/culture medium and 3-minute ultrasonication resulted in a well-dispersed medium with dispersion and isolation of agglomerated MWCNT by ultrasonication which manifested the highest cytotoxicity. The cytotoxicity was more potent for chrysotile than MWCNT. The genotoxicity of MWCNT was characterized by the formation of polyploidy without structural chromosome aberration, and an increased number of bi- and multi-nucleated cells without micronucleus induction, as well as negative hgprt mutagenicity. Chrysotile exhibited essentially the same genotoxicity as MWCNT, except for marginal but significant induction of micronuclei. MWCNT and chrysotile were incompletely internalized in the cells and localized in the cytoplasm. Conclusions: MWCNT and chrysotile were cytotoxic and genotoxic in Chinese hamster lung cells, but might interact indirectly with DNA. The results suggest that both test substances interfere physically with biological processes during cytokinesis. (J Occup Health 2010; 52: 155-166)