Uncoupler of oxidative phosphorylation prolongs the lifespan of Drosophila
Uncoupler of oxidative phosphorylation prolongs the lifespan of Drosophila
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DOI：
10.1007/s10541-005-0213-1
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发表时间：
2005-09-01
影响因子：
2.8
通讯作者：
Padalko, VI
中科院分区：
文献类型：
--
作者：
Padalko, VI
研究了线粒体中度（“软”）解偶联对俄勒冈州果蝇品系的寿命和生物年龄的一些参数的影响。在幼虫的营养混合物中添加解偶联剂 2,4-二硝基苯酚 (DNP) 可显着延长果蝇的平均寿命，而不会改变其最大寿命。 DNP 显着增加了状态 4（机会）果蝇的分离线粒体和组织匀浆的耗氧率。 DNP 还降低了组织匀浆中乙醇脱氢酶（果蝇生物学年龄的参数）的活性，特别是在辛醇作为反应底物的情况下。然而，与对照果蝇相比，DNP 处理果蝇的食物被剥夺后，其生存能力显着下降。总的来说，结果表明线粒体的“软”解偶联可能会延长寿命。
The effect of a moderate ("soft") uncoupling of mitochondria on the lifespan and some parameters of biological age of Drosophila melanogaster strain Oregon was studied. Addition of the uncoupler 2,4-dinitrophenol (DNP) to the nutritional mixture of larvae significantly increased the average lifespan of the flies without changing their maximal lifespan. DNP significantly increased the rate of oxygen consumption by isolated mitochondria and tissue homogenates of the flies in state 4 (of Chance). DNP also decreased the activity of alcohol dehydrogenase (a parameter of flies' biological age) in the tissue homogenates, especially on octanol as the reaction substrate. However, being deprived of food the DNP-treated flies displayed a markedly decreased viability as compared to the control flies. On the whole, the results suggest that "soft" uncoupling of mitochondria may increase the lifespan.