Improved normal tissue protection by proton and X-ray microchannels compared to homogeneous field irradiation
Improved normal tissue protection by proton and X-ray microchannels compared to homogeneous field irradiation
复制标题
DOI：
10.1016/j.ejmp.2015.04.004
复制
发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Wilkens, J. J.
中科院分区：
文献类型：
--
作者：
Girst, S.;Marx, C.;Wilkens, J. J.
发展正常组织损伤的风险通常限制了在放射治疗中可以应用于肿瘤的放射剂量。微束放射治疗（MRT）是一种空间分割光子放射治疗，目前正在欧洲同步辐射设施（ESRF）进行测试，以改善正常组织的保护。MRT利用由同步加速器产生的显微镜下薄且几乎平行的X射线束阵列。在慕尼黑的离子微探针SNAKE上，研究了聚焦质子微束（“质子微通道”）以改善正常组织保护。在这里，我们比较研究微束/微通道照射与亚毫米X射线与质子束，以尽量减少正常组织损伤的风险，在人体皮肤模型，在体外。皮肤组织照射的平均剂量为2戈伊的照射区域，在ESRF平行同步加速器产生的X射线束或与20 MeV的质子在SNAKE使用四种不同的照射模式：均匀场，平行线和微通道应用程序，使用两种不同的通道尺寸。在MTT试验中测定的正常组织活力在质子或X射线微通道照射后显著高于均匀场照射。与这些发现一致，遗传损伤，如通过测量角质形成细胞中的微核所确定的，与均匀场照射相比，质子或X射线微通道后显着减少。我们的数据表明，与均匀照射相比，使用X射线或质子微通道的皮肤照射保持更高的细胞活力和DNA完整性，从而可能改善放射治疗后的正常组织保护。(C)2015年意大利医学协会。爱思唯尔有限公司出版
The risk of developing normal tissue injuries often limits the radiation dose that can be applied to the tumour in radiation therapy. Microbeam Radiation Therapy (MRT), a spatially fractionated photon radiotherapy is currently tested at the European Synchrotron Radiation Facility (ESRF) to improve normal tissue protection. MRT utilizes an array of microscopically thin and nearly parallel X-ray beams that are generated by a synchrotron. At the ion microprobe SNAKE in Munich focused proton microbeams ("proton microchannels") are studied to improve normal tissue protection. Here, we comparatively investigate microbeam/microchannel irradiations with sub-millimetre X-ray versus proton beams to minimize the risk of normal tissue damage in a human skin model, in vitro. Skin tissues were irradiated with a mean dose of 2 Gy over the irradiated area either with parallel synchrotron-generated X-ray beams at the ESRF or with 20 MeV protons at SNAKE using four different irradiation modes: homogeneous field, parallel lines and microchannel applications using two different channel sizes. Normal tissue viability as determined in an MTT test was significantly higher after proton or X-ray microchannel irradiation compared to a homogeneous field irradiation. In line with these findings genetic damage, as determined by the measurement of micronuclei in keratinocytes, was significantly reduced after proton or X-ray microchannel compared to a homogeneous field irradiation. Our data show that skin irradiation using either X-ray or proton microchannels maintain a higher cell viability and DNA integrity compared to a homogeneous irradiation, and thus might improve normal tissue protection after radiation therapy. (C) 2015 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd.