Prostate-specific membrane antigen (PSMA) protein expression in normal and neoplastic tissues and its sensitivity and specificity in prostate adenocarcinoma: an immunohistochemical study using mutiple tumour tissue microarray technique
Prostate-specific membrane antigen (PSMA) protein expression in normal and neoplastic tissues and its sensitivity and specificity in prostate adenocarcinoma: an immunohistochemical study using mutiple tumour tissue microarray technique
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DOI：
10.1111/j.1365-2559.2007.02635.x
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发表时间：
2007-03-01
期刊：
影响因子：
6.4
通讯作者：
Penetrante, R.
中科院分区：
文献类型：
--
作者：
Mhawech-Fauceglia, P.;Zhang, S.;Penetrante, R.
目的：确定正常组织和 3161 例良性和恶性肿瘤中前列腺特异性膜抗原 (PSMA) 的表达，并随后确定其在前列腺腺癌 (PaC) 中的敏感性和特异性。方法和结果：用 PSMA 单克隆抗体对多个组织微阵列切片进行染色。 93/141 例 (66.0%) 的 PaC 呈阳性，具有多种染色模式，包括细胞质、顶端、顶端/细胞质和细胞质伴膜强化。在 2174 种不同的肿瘤类型中，154 种表达 PSMA，其中包括 59/346 (17.0%) 膀胱尿路上皮癌 (UBC)。在这些肿瘤中，染色模式始终是细胞质的。所有 846 个良性肿瘤的 PSMA 均为阴性。 PSMA 区分 PaC 与任何其他类型恶性肿瘤的敏感性和特异性分别为 65.9% 和 94.5%。此外，其区分 PaC 与尿路上皮癌的敏感性和特异性分别为 65.9% 和 82.9%。结论：尽管 PSMA 在各种类型的恶性肿瘤亚型中表达，但仍被认为对 PaC 具有相当的敏感性和高度特异性。
Aims: To determine prostate-specific membrane antigen (PSMA) expression in normal tissues and in 3161 benign and malignant tumours and subsequently to define its sensitivity and specificity in prostatic adenocarcinoma (PaC).Methods and results: Multiple tissue microarray sections were stained with a monoclonal antibody to PSMA. PaC was positive in 93/141 cases (66.0%) with various staining patterns including cytoplasmic, apical, apical/cytoplasmic and cytoplasmic with membranous accentuation. Of 2174 various tumour types, 154 expressed PSMA, including 59/346 (17.0%) urothelial carcinomas of the bladder (UBC). In those tumours, the staining pattern was always cytoplasmic. All 846 benign tumours were negative for PSMA. The sensitivity and specificity of PSMA in distinguishing PaC from any other type of malignancy is 65.9% and 94.5%, respectively. Furthermore, its sensitivity and specificity in differentiating PaC from urothelial cancer is 65.9% and 82.9%, respectively.Conclusions: Despite its expression by subsets of various types of malignancies, PSMA is still considered to be fairly sensitive and highly specific for PaC.