Internucleosomal Interactions Mediated by Histone Tails Allow Distant Communication in Chromatin
Internucleosomal Interactions Mediated by Histone Tails Allow Distant Communication in Chromatin
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DOI：
10.1074/jbc.m111.333104
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发表时间：
2012-06-08
影响因子：
4.8
通讯作者：
Olson, Wilma K.
中科院分区：
文献类型：
--
作者：
Kulaeva, Olga I.;Zheng, Guohui;Olson, Wilma K.
染色质上的长距离作用是真核生物转录调控的标志。虽然染色质结构本身可以支持长距离相互作用，但染色质中广泛分布的DNA模块之间的有效通信机制仍然是一个谜。本文描述的分子模拟表明，瞬时二元核小体间相互作用可以介导染色质中的远距离通信。核心组蛋白的N-末端尾部和DNA之间的静电相互作用增强了计算出的沿着DNA序列沿着相距很远的位点并置的概率。在染色质结构的通信速率的实验分析证实，长距离通信有效地发生，独立于含尾，但不是无尾，染色质上的距离。两者合计，我们的数据表明，涉及组蛋白尾部的核小体间相互作用对于真核基因组中调控元件及其靶点之间的高效、长距离通信至关重要。
Action across long distances on chromatin is a hallmark of eukaryotic transcriptional regulation. Although chromatin structure per se can support long-range interactions, the mechanisms of efficient communication between widely spaced DNA modules in chromatin remain a mystery. The molecular simulations described herein suggest that transient binary internucleosomal interactions can mediate distant communication in chromatin. Electrostatic interactions between the N-terminal tails of the core histones and DNA enhance the computed probability of juxtaposition of sites that lie far apart along the DNA sequence. Experimental analysis of the rates of communication in chromatin constructs confirms that long-distance communication occurs efficiently and independently of distance on tail-containing, but not on tailless, chromatin. Taken together, our data suggest that internucleosomal interactions involving the histone tails are essential for highly efficient, long-range communication between regulatory elements and their targets in eukaryotic genomes.