The majority of human glutathione peroxidase type 5 (GPX5) transcripts are incorrectly spliced: implications for the role of GPX5 in the male reproductive tract
The majority of human glutathione peroxidase type 5 (GPX5) transcripts are incorrectly spliced: implications for the role of GPX5 in the male reproductive tract
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DOI：
10.1042/bj3330005
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发表时间：
1998-07-01
影响因子：
4.1
通讯作者：
Jury, JA
中科院分区：
文献类型：
--
作者：
Hall, L;Williams, K;Jury, JA
此前已提出一种附睾特异性分泌型谷胱甘肽过氧化物酶(GPX5)，用于保护哺乳动物精子膜免受脂质过氧化的有害影响，如果不加以控制，可能会导致受精能力下降。在这里，我们报道了人GPX5基因的克隆，并表明大多数转录本包含118个核苷酸移码缺失，很可能是由于在加工过程中不适当地切除了外显子3。针对重组人GPX5提出的抗血清与大鼠和猕猴(猕猴)附睾蛋白发生交叉反应，其大小与预期的全长、活性GPX5的大小相同。然而，在任何被测试的人体样本中都没有显示出类似的反应。
An epididymis-specific, secretory glutathione peroxidase (GPX5) has been proposed previously to play a role in protecting mammalian sperm membranes from the deleterious effects of lipid peroxidation, which, if not contained, can lead to reduced fertilizing capacity. Here we report the cDNA cloning of human GPX5 and show that the majority of transcripts contain a 118 nt frame-shifting deletion, arising, most likely, from inappropriate excision of exon 3 during processing. Antisera raised against recombinant human GPX5 cross-reacted with rat and macaque (Macaca fascicularis) epididymal proteins of the size expected for full-length, active GPX5. However, no similar reactivity could be demonstrated in any of the human samples tested.