Optical coherence tomography of enucleated human eye specimens with histological correlation: Origin of the outer "red line"
Optical coherence tomography of enucleated human eye specimens with histological correlation: Origin of the outer "red line"
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DOI：
10.1016/j.ajo.2005.10.019
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发表时间：
2006-04-01
影响因子：
4.2
通讯作者：
Gehlbach, PL
中科院分区：
文献类型：
--
作者：
Ghazi, NG;Dibernardo, C;Gehlbach, PL
目的：进一步明确视网膜光学相干断层扫描图像中“外红线”（ORL）的来源。“方法：研究了威尔默眼科研究所先前因脉络膜黑色素瘤摘除的7只人眼的11个福尔马林固定帽。ORL评价包括在单独但相邻的区域连续手术清除视网膜色素上皮、布鲁赫膜（BM）和脉络膜。通过这些区域的光学相干断层扫描（OCT）图像与相应的组织切片相关。视网膜色素上皮，BM，脉络膜的ORL在视网膜OCT 3 images的相对贡献进行了evaluated.RESULTS：在检查的标本，ORL相关的解剖结构与高度反射光学表面的水平外视网膜，并没有出现代表一个特定的解剖层。反射层似乎由视网膜色素上皮、BM和内部脉络膜组成。BM和内部脉络膜对ORL的相对贡献似乎大于视网膜色素上皮。该研究表明，存在于视网膜OCT 3图像中并且通常与视网膜色素上皮/BM/内部脉络膜复合体相关的ORL似乎指示高度反射的光学表面，当眼睛层正常配置时，代表了这种解剖结构的复合体，其中BM和内部脉络膜起主要作用。这项研究的结果表明，OCT在眼科病理学实验室的潜在作用。
PURPOSE: To further define the origin of the "outer red line" (ORL) present in retinal optical coherence tomograms.DESIGN: Laboratory investigation. "METHODS: Eleven formalin-fixed caps of seven human eyes previously enucleated for choroidal melanoma at the Wilmer Eye Institute were studied. The ORL evaluation consisted of sequential, surgical elimination of the retinal pigment epithelium, Bruch's membrane (BM), and choroid in separate, but adjacent, areas. Optical coherence tomography (OCT) images through each of these areas were correlated with corresponding histologic sections. The relative contribution of the retinal pigment epithelium, BM, and choroid to the ORL in retinal OCT3 images was evaluated.RESULTS: In the specimens examined, the ORL correlated anatomically with a highly reflective optical surface at the level of the outer retina, and did not appear to represent a specific anatomic layer. The reflecting layers appeared to be composed of the retinal pigment epithelium, BM, and inner choroid. The relative contribution of BM together with the inner choroid to the ORL appeared greater than that of the retinal pigment epithelium.CONCLUSIONS: This study suggests that the ORL present in retinal OCT3 images and generally associated with the retinal pigment epithelium/BM/inner choroidal complex appears to indicate a highly reflective optical surface which, when eye layers are normally configured, represents this complex of anatomic structures with a predominant contribution from BM and inner choroid. The findings of this study suggest a potential role for OCT in the eye pathology laboratory.