Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli
Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli
复制标题
DOI：
10.1038/289555a0
复制
发表时间：
1981-02
期刊：
影响因子：
64.8
通讯作者：
H. Küpper;W. Keller;C. Kurz;S. Forss;H. Schaller;R. Franze;K. Strohmaier;O. Marquardt;V. Zaslavsky;P. Hofschneider
中科院分区：
文献类型：
--
作者：
H. Küpper;W. Keller;C. Kurz;S. Forss;H. Schaller;R. Franze;K. Strohmaier;O. Marquardt;V. Zaslavsky;P. Hofschneider
将口蹄疫病毒单链基因组RNA的双链DNA克隆到大肠杆菌表达载体pBR322中。建立了病毒基因组的限制性内切酶图谱，并与口蹄疫病毒的生化图谱进行了比对。鉴定了病毒主要抗原结构蛋白Vp1的编码序列，并将其插入到由噬菌体λ启动子控制表达的载体中。在合适的宿主中，用放射免疫法可以证明抗原多肽的合成。
Double-stranded DNA copies of the single-stranded genomic RNA of foot and mouth disease virus have been cloned into the Escherichia coli plasmid pBR322. A restriction map of the viral genome was established and aligned with the biochemical map of foot and mouth disease virus. The coding sequence for structural protein VP1, the major antigen of the virus, was identified and inserted into a plasmid vector where the expression of this sequence is under control of the phage λ PLpromoter. In an appropriate host the synthesis of antigenic polypeptide can be demonstrated by radioimmunoassay.