Purification of the flavin-containing monooxygenase from mouse and pig liver microsomes.
Purification of the flavin-containing monooxygenase from mouse and pig liver microsomes.
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从小鼠和猪肝微粒体中纯化含黄素单加氧酶。
DOI：
10.1016/0020-711x(84)90180-0
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Hodgson,E
中科院分区：
文献类型：
--
作者：
Sabourin,PJ;Smyser,BP;Hodgson,E
用Cibacron Blue Sepharose、Procion Red琼脂糖和2 '5'-ADP Sepharose从小鼠和猪肝中纯化了含黄素的微粒体单加氧酶。酶的最终比活性分别为1200和954 nmol/min/mg蛋白质从小鼠和猪肝。变性聚丙烯酰胺凝胶电泳显示，小鼠和猪肝中的酶均具有典型的黄素蛋白谱。
The microsomal flavin-containing monooxygenase has been purified from mouse and pig liver utilizing Cibacron-Blue Sepharose, Procion-Red agarose, and 2'5'-ADP Sepharose. The enzymes had a final specific activity of 1200 and 954 nmol/min/mg protein from mouse and pig liver respectively. The enzyme from both mouse and pig liver displayed typical flavoprotein spectra and appeared homogeneous by denaturing polyacrylamide gel electrophoresis.