THE ELECTROMOTIVE ACTION OF ACETYLCHOLINE AT THE MOTOR END-PLATE
THE ELECTROMOTIVE ACTION OF ACETYLCHOLINE AT THE MOTOR END-PLATE
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DOI：
10.1113/jphysiol.1950.sp004492
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发表时间：
1950-01-01
影响因子：
5.5
通讯作者：
FATT, P
中科院分区：
文献类型：
--
作者：
FATT, P
方法制备。各种骨骼肌的青蛙，蛙temporaria，使用。分枝腹直肌和肌缝匠肌作为初步实验中的实验材料，其中仅记录机械响应。为了记录电势变化，m。Sartorius和M.使用第四趾长伸肌。对于那些采用电记录的实验，在解剖时必须特别小心。只有少数纤维的可见损伤导致损伤区域出现明显的负性。所有这些受伤的肌肉都必须被丢弃。在冬季和夏季青蛙身上获得的结果似乎没有什么不同。实验在室温下进行，在14和210 ℃之间变化。设备.用缝匠肌同时记录电和机械变化的实验装置已经描述过（Fatt，1949）。在所有其他的实验中，肌肉都是垂直安装在一个玻璃腔中，这样的结构可以通过容器底部的一个连接器来更换沐浴液。通过将连接到肌肉一端肌腱的线连接到等张杠杆来记录机械响应。
METHOD Preparation. Various skeletal muscles of the frog, Rana temporaria, were used. The m. rectus abdominis and m. sartorius served as the experimental material in preliminary experiments in which mechanical responses alone were recorded. For recording electric potential changes the m. sartorius and m. extensor longus digiti quarti were used. Extreme care had to be taken in the dissection for those experiments in which electrical recording was employed. Visible damage of only a few fibres caused an appreciable negativity to appear at the injured region. All such injured muscles had to be discarded.There appeared to be no difference in the results obtained with winter and summer frogs. The experiments were carried out at room temperature, varying between 14 and 210 C. Apparatus. The experimental arrangement employed with the sartorius for the simultaneous recording of electrical and mechanical changes has already been described (Fatt, 1949). In all other experiments the muscle was mounted vertically in a glass chamber so constructed that bathingsolutions could be changed through a connexion at the bottom of the vessel. The mechanical response was recorded by attaching the thread tied to the tendon at one end of the muscle to an isotonic lever.