Differential alterations of the concentrations of endocannabinoids and related lipids in the subcutaneous adipose tissue of obese diabetic patients
Differential alterations of the concentrations of endocannabinoids and related lipids in the subcutaneous adipose tissue of obese diabetic patients
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DOI：
10.1186/1476-511x-9-43
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发表时间：
2010-04-28
影响因子：
4.5
通讯作者：
Di Marzo, Vincenzo
中科院分区：
文献类型：
--
作者：
Annuzzi, Giovanni;Piscitelli, Fabiana;Di Marzo, Vincenzo
背景资料：内源性大麻素，大麻素和2-AG，由脂肪细胞产生，在那里它们通过大麻素CB 1受体刺激脂肪生成，并在瘦素和胰岛素的负控制下。内源性大麻素水平在肥胖个体和非肥胖2型糖尿病患者的血液中升高。迄今为止，没有研究评估患有肥胖和2型糖尿病（OBT 2D）的受试者的皮下脂肪组织（SAT）中的内源性大麻素水平，所述受试者的特征在于与非糖尿病肥胖受试者（OB）相比相似的肥胖和全身胰岛素抵抗以及较低的血浆瘦素水平。在10例OBT 2D，11例OB，和8名正常体重的非糖尿病受试者，用液相色谱-质谱法测定。结果：与NW相比，SAT中花生四烯酸、OEA和PEA水平升高2-4.4倍（p < 0.05），而2-AG水平降低2.3倍（p < 0.05），但SAT中花生四烯酸、OEA和PEA水平均无显著性差异。
Background: The endocannabinoids, anandamide and 2-AG, are produced by adipocytes, where they stimulate lipogenesis via cannabinoid CB1 receptors and are under the negative control of leptin and insulin. Endocannabinoid levels are elevated in the blood of obese individuals and nonobese type 2 diabetes patients. To date, no study has evaluated endocannabinoid levels in subcutaneous adipose tissue (SAT) of subjects with both obesity and type 2 diabetes (OBT2D), characterised by similar adiposity and whole body insulin resistance and lower plasma leptin levels as compared to non-diabetic obese subjects (OB).Design and Methods: The levels of anandamide and 2-AG, and of the anandamide-related PPAR alpha ligands, oleoylethanolamide (OEA) and palmitoylethanolamide (PEA), in the SAT obtained by abdominal needle biopsy in 10 OBT2D, 11 OB, and 8 non-diabetic normal-weight (NW) subjects, were measured by liquid chromatography-mass spectrometry. All subjects underwent a hyperinsulinaemic euglycaemic clamp.Results: As compared to NW, anandamide, OEA and PEA levels in the SAT were 2-4.4-fold elevated (p < 0.05), and 2-AG levels 2.3-fold reduced (p