The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene
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转录因子与 5S 核糖体 RNA 基因缺失突变体的结合
DOI：
10.1016/0092-8674(81)90429-3
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发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
R. Roeder
中科院分区：
文献类型：
--
作者：
S. Sakonju;Donald D. Brown;D. Engelke;S. Ng;B. S. Shastry;R. Roeder
转录因子特异性结合到5s核糖体RNA基因内的一个区域，已知该区域是基因转录准确起始所必需的。我们通过比较各种缺失突变体启动转录和结合蛋白质的能力，证实了这种相互作用的重要性。当基因从3 '方向上删除+ 83以上时，蛋白质的特异性结合就会丧失。这恰好与启动转录能力的丧失相吻合。相反，从5 ‘方向进入基因并延伸到控制区5 ’边界以外的缺失（+ 50/+ 55）对控制区的其余部分提供了部分保护。这一观察结果可以解释为什么在控制区5 '边界以外不能启动转录的缺失仍然可以竞争野生型5s RNA基因的转录。
A transcription factor binds specifically to a region within the 5s ribosomal RNA gene that is known to be required for accurate initiation of transcription of the gene. We have confirmed the significance of this interaction by comparing various deletion mutants for their ability to initiate transcription and to bind the protein. Loss of specific binding of the protein occurs when the gene is deleted beyond+ 83 from the 3’direction. This coincides exactly with the loss of ability to initiate transcription. In contrast, deletions entering the gene from the 5’direction and extending beyond the 5’border of the control region (+ 50/+ 55) give partial protection of the remainder of the control region. This observation can explain why deletions beyond the 5’border of the control region that are unable to initiate transcription can still compete for the transcription of the wild-type 5s RNA gene.