ENDOTHELIAL-CELL INDUCED MODULATION OF CARDIAC FIBROBLAST COLLAGEN-METABOLISM
ENDOTHELIAL-CELL INDUCED MODULATION OF CARDIAC FIBROBLAST COLLAGEN-METABOLISM
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DOI：
10.1093/cvr/27.6.1004
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发表时间：
1993-06-01
影响因子：
10.8
通讯作者：
WEBER, KT
中科院分区：
文献类型：
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作者：
GUARDA, E;MYERS, PR;WEBER, KT
目的：观察共培养条件下血管内皮细胞对大鼠心脏成纤维细胞胶原合成及胶原酶活性的影响，并探讨血管紧张素和醛固酮是否影响内皮细胞诱导成纤维细胞胶原代谢的调节。方法：将牛主动脉内皮细胞培养在微孔膜插入物上融合，然后与从成年大鼠获得的心脏成纤维细胞共培养，之前在添加0.4%胎牛血清的35 mm培养皿中培养。h -3-脯氨酸掺入法测定胶原合成。实验也在类似的条件下进行，用酶谱法在条件培养基中定量测定成纤维细胞与内皮细胞共培养的胶原酶活性。结果：共培养48小时后，成纤维细胞的胶原合成比单独培养时增加了1.9倍(p
Objective: The aim was to examine the influence of vascular endothelial cells on rat cardiac fibroblast collagen synthesis and collagenase activity under co-culture conditions, and to determine whether angiotensin II or aldosterone influence endothelial cell induced modulation of fibroblast collagen metabolism. Methods: Bovine aortic endothelial cells were grown to confluence on microporous membrane inserts and then co-cultured with cardiac fibroblasts obtained from adult rats, previously grown to confluence in 35 mm dishes supplemented with 0.4% fetal bovine serum. Collagen synthesis was measured by H-3-proline incorporation. Experiments were also conducted under similar conditions to quantitate collagenase activity by zymography in conditioned medium from fibroblasts co-cultured with endothelial cells. Results: After 48 h co-culture, there was a 1.9-fold increase in fibroblast collagen synthesis when compared to fibroblasts alone (p