Effects of Zuogui Pill (sic) on Wnt Singal Transduction in Rats with Glucocorticoid-induced Osteoporosis
Effects of Zuogui Pill (sic) on Wnt Singal Transduction in Rats with Glucocorticoid-induced Osteoporosis
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DOI：
10.1016/s0254-6272(11)60020-4
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发表时间：
2011-06-01
影响因子：
2.6
通讯作者：
Ju Da-hong
中科院分区：
文献类型：
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作者：
Liu Mei-jie;Li Yan;Ju Da-hong
目的：从Wnt信号转导通路的角度揭示左归丸治疗激素性骨质疏松症的作用机制，为进一步阐明中医“肾主骨”的科学内涵提供实验依据。选用雄性Wistar大鼠42只，随机分为3组，对照组（n=12）、模型组（n=15）和左归丸组（n=15）。先用地塞米松注射8周制作骨质疏松模型，左归丸组用左归丸灌胃8周。在不脱钙的胫骨切片中测量相关的形态学参数。结果：与对照组相比，模型组大鼠胫骨成骨细胞（OB）和骨髓基质细胞（BMC）TBV%和TFS%显著降低，TRS%显著升高，Wnt 1、LRP-5和beta-catenin蛋白表达显著降低。结论：左归丸可通过上调Wnt信号转导通路中关键信号分子Wnt 1、LRP-5和beta-catenin的表达，防治激素性骨质疏松症。
Objective: To reveal the mechanism of Zuogui Pill (sic) in treatment of glucocorticoid-induced osteoporosis from the angle of the Wnt signal transduction pathway and to provide further experimental evidence for expounding the scientific connotation of "the kidney dominating the bones" in TCM.Methods: Forty-two male Wistar rats were selected and randomly divided into three groups, control group (n=12), model group (n=15) and Zuogui Pill group (n=15). Form the beginning, The rats were injected dexamethasone for eight weeks to make the model of osteoporosis, and the Zuogui Pill were administered intragastrically to the rats of Zuogui Pill group for eight weeks. The relative morphological parameters were measured in the undecalcified tibial slices. And the protein expression levels of Wnt1, LRP-5 and beta-catenin in rat tibial osteoblasts (OB) and bone marrow stromal cells (BMC) were detected by immunohistochemistry.Results: Compared with the control group, TBV% and TFS% decreased significantly, while TRS% increased significantly, and the protein expression of Wnt1, LRP-5 and beta-catenin in OB and BMC decreased significantly in the model group. And compared with the model group, TBV% and TFS% increased significantly, and expression levels of Wnt1, LRP-5 and beta-catenin proteins increased significantly in the Zuogui pill group.Conclusion: Zuogui Pill can prevent and treat glucocorticoid-induced osteoporosis in rats by up-regulating the expression of the key signal molecules Wnt1, LRP-5 and beta-catenin in Wnt signal transduction pathway.