Facile and Efficient Construction of Water‐Soluble Biomaterials with Tunable Mesoscopic Structures Using All‐Natural Edible Proteins
Facile and Efficient Construction of Water‐Soluble Biomaterials with Tunable Mesoscopic Structures Using All‐Natural Edible Proteins
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DOI：
10.1002/adfm.201901830
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发表时间：
2019-04
影响因子：
19
通讯作者：
Tao Wang;Xianfu Chen;Q. Zhong;Zhengxing Chen;Ren Wang;Ashok R. Patel
中科院分区：
文献类型：
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作者：
Tao Wang;Xianfu Chen;Q. Zhong;Zhengxing Chen;Ren Wang;Ashok R. Patel
使用天然分子开发具有可调化学和物理性质的生物材料是广泛应用的核心，但对可定制结构的生物大分子结构的操纵仍然有限。在本文中，首次提出了一组全天然的、无处不在的基于可食用蛋白质的纳米结构，其介观结构具有迷人的可调性，易于从3D纳米网转化为2D薄片和纳米颗粒。这是通过简单地将酪蛋白胶束和各种量的大米蛋白溶解在碱性溶液（pH 12）中，然后中和以形成介观蛋白质复合物来实现的。通过原子力显微镜、低温透射电子显微镜和X射线衍射证实了调整两种蛋白质比例所导致的结构转变。当装载疏水模型药物芹菜素（也是一种食物来源的分子）时，介观结构决定了芹菜素的体内抗肿瘤活性。所研究材料的可用性和生物相容性，加上简单的制备和易于操作的介观结构，在设计具有众多应用的按需生化运载材料方面有希望。
Developing biomaterials with tunable chemical and physical properties using natural molecules is central to a wide range of applications, yet the manipulation of biomacromolecular structures toward tailorable architectures remains limited. Herein, for the first time, a set of all‐natural, ubiquitous edible protein‐based nanostructures with fascinating tunability in their mesostructures easily transformed from 3D nanonet to 2D thin sheet and to nanoparticles is presented. This is achieved by simply dissolving casein micelles and various amounts of rice proteins in an alkaline solution (pH 12), followed by neutralization to form mesoscale protein composites. The structural transitions as a result of tuning the two protein ratio are confirmed by atomic force microscopy, cryo‐transmission electron microscopy, and X‐ray diffraction. When loaded with a hydrophobic model drug, apigenin (also a food‐sourced molecule), the mesoscopic structure determines the in vivo anti‐tumor activity of apigenin. The availability and biocompatibility of the studied materials, plus simple preparation and easy manipulation of the mesostructures, have promise in designing on‐demand biochemical‐carrying materials with numerous applications.