Accurate rocking-curve measurements on protein crystals grown in a homogeneous magnetic field of 2.4 T
Accurate rocking-curve measurements on protein crystals grown in a homogeneous magnetic field of 2.4 T
复制标题
DOI：
10.1107/s0907444904005268
复制
发表时间：
2004-06-01
影响因子：
2.2
通讯作者：
Weckert, E
中科院分区：
文献类型：
--
作者：
Lübbert, D;Meents, A;Weckert, E
通过X射线衍射摇摆曲线测量，研究了在2.4 T均匀磁场内外生长的溶菌酶晶体之间的镶嵌性差异，以及有无琼脂糖凝胶。使用Si（113）四反射Bartels单色器实现了高角分辨率。结果表明，（i）所有晶体都是高度完美的，（ii）镶嵌性明显是各向异性的，（iii）镶嵌性在每组晶体（在相同条件下生长）中的变化比各组之间的平均值更强烈。特别地，发现磁场对晶体镶嵌性的影响非常小。最后，蛋白质晶体内部的马赛克块的空间分布可视化与一种新的衍射技术，使用高空间分辨率的二维CCD检测器。
Differences in mosaicity between lysozyme crystals grown inside and outside a homogeneous magnetic field of 2.4 T and with and without agarose gel were investigated by X-ray diffraction rocking-curve measurements. High angular resolution was achieved using an Si( 113) four-reflection Bartels monochromator. The results show that (i) all crystals were highly perfect, (ii) the mosaicities were clearly anisotropic and (iii) the mosaicities varied more strongly within each group of crystals ( grown under identical conditions) than the average values across groups. In particular, the effect of the magnetic field on crystal mosaicity was found to be very small. Finally, the spatial distribution of mosaic blocks inside a protein crystal was visualized with a novel diffraction technique using a high spatial resolution two-dimensional CCD detector.