Stellate cells in the human vocal fold
Stellate cells in the human vocal fold
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DOI：
10.1177/000348940111000405
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发表时间：
2001-04-01
影响因子：
1.4
通讯作者：
Nakashima, T
中科院分区：
文献类型：
--
作者：
Sato, K;Hirano, M;Nakashima, T
在成人声带粘膜中发现了星形细胞，并能主动合成细胞外基质。这些细胞没有命名，因此在本研究中被命名为声带星状细胞（VFSC）。在离体成人喉标本上，对人声带粘膜中的VFSC进行了光镜和电镜观察。将VFSC与常规成纤维细胞进行了比较。结果总结如下。1)VFSC分布于成人黄斑区。2)VFSC形状不规则，呈星状，胞质突起细长。3)脂滴存在于细胞质中。4)VFSC具有小的核质比和发达的粗面内质网，表明这些细胞中蛋白质合成活跃。5)无基膜存在，在细胞质中可以看到细丝。6)VFSC细胞浆内高碘酸-希夫染色和III型胶原染色较强。7)VFSC积极合成胶原纤维，包括网状纤维，以及其他细胞外基质，如弹性纤维和糖胺聚糖（透明质酸）。8)VFSC。本研究首次证实，在正常条件下，人成人声带粘膜中的细胞外基质可主动合成。9)VFSC参与细胞外基质的代谢，细胞外基质对于人成人声带粘膜固有层作为振动组织的粘弹性特性至关重要。
Cells have been discovered that are star-like in appearance and that actively synthesize extracellular matrices in the human adult vocal fold mucosa. These cells have no nomenclature and are thus designated as vocal fold stellate cells (VFSC) in this study. Light and electron microscopic investigation of VFSC in the human vocal fold mucosa was carried out on excised human adult larynges. A comparison between VFSC and conventional fibroblasts was made. The results are summarized as follows. 1)The VFSC are distributed in human adult maculae flavae. 2) The VFSC are irregular and stellate in shape, possessing slender cytoplasmic processes. 3) Lipid droplets are present in the cytoplasm. 4) The VFSC have a small nucleus-cytoplasm ratio and well-developed rough endoplasmic reticulum, suggesting active protein synthesis in these cells. 5) No basal lamina is present, and filaments can be seen in the cytoplasm. 6) The VFSC show strong cytoplasm staining with periodic acid-Schiff stain and type III collagen. 7) The VFSC actively synthesize collagenous fibers, including reticular fibers, as well as other extracellular matrices, such as elastic fibers and glycosaminoglycan (hyaluronic acid). 8) The VFSC. first demonstrated in this study, actively synthesize extracellular matrices in the human adult vocal fold mucosa under normal conditions. 9) The VFSC participate in the metabolism of the extracellular matrices essential for the viscoelastic properties of the lamina propria of the human adult vocal fold mucosa as a vibrating tissue.