The EAST protein of Drosophila controls an expandable nuclear endoskeleton
The EAST protein of Drosophila controls an expandable nuclear endoskeleton
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DOI：
10.1038/35010535
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发表时间：
2000-05-01
影响因子：
21.3
通讯作者：
Chia, W
中科院分区：
文献类型：
--
作者：
Wasser, M;Chia, W
核内高度有序的结构表明存在内部核骨架。我们对果蝇east基因的研究，以幼虫唾液腺多线核为模型，证明了染色体外核结构参与调节核结构。EAST是一种新的普遍存在的蛋白质，是east（增强的成人感觉阈值）位点的产物，定位于细胞核的染色体外结构域。EAST的核水平增加，以响应热休克。核EAST的增加，无论是由热休克或转基因过表达引起的，导致EAST标记的染色体外结构域的扩展，伴随着染色体之间的间距的增加。此外，EAST的功能，以促进优先积累的蛋白质CP 60和肌动蛋白在染色体外区域的核。我们建议，EAST介导的可膨胀的核内骨骼，通过改变其体积，可以调节染色体的空间排列的组装。
The high degree of structural order inside the nucleus suggests the existence of an internal nucleoskeleton. Our studies on the east gene of Drosophila, using the larval salivary gland polytene nucleus as a model, demonstrate the involvement of an extrachromosomal nuclear structure in modulating nuclear architecture. EAST, a novel ubiquitous protein, the product of the east (enhanced adult sensory threshold) locus, is localized to an extrachromosomal domain of the nucleus. Nuclear levels of EAST are increased in response to heat shock. Increase in nuclear EAST, whether caused by heat shock or by transgenic overexpression, results in the expansion of the extrachromosomal domain labelled by EAST, with a concomitant increase in the spacing between chromosomes. Moreover, EAST functions to promote the preferential accumulation of the proteins CP60 and actin in extrachromosomal regions of the nucleus. We propose that EAST mediates the assembly of an expandable nuclear endoskeleton which, through alterations of its volume, can modulate the spatial arrangement of chromosomes.