Direct interaction between CCN family protein 2 and fibroblast growth factor 1
Direct interaction between CCN family protein 2 and fibroblast growth factor 1
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DOI：
10.1007/s12079-014-0232-z
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发表时间：
2014-06-01
影响因子：
4.1
通讯作者：
Takigawa, Masaharu
中科院分区：
文献类型：
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作者：
El Kader, Tarek Abd;Kubota, Satoshi;Takigawa, Masaharu
CCN家族蛋白2（CCN family protein 2，CCN 2）是一种具有多种功能的基质细胞蛋白，为了寻找CCN家族蛋白2的新的分子对应物，我们进行了相互作用组学分析，发现成纤维细胞生长因子（fibroblast growth factor，FGF）-1是候选者之一。固相结合试验表明CCN 2与FGF-1之间具有特异性结合。这种结合也通过表面等离子体共振（SPR）分析证实，其显示解离常数（Kd）为3.98 nM，表明两者之间的强分子相互作用。RNA分析表明，FGF-1和CCN 2都可以由软骨细胞产生，因此它们在软骨中的相互作用是可能的。这些发现首次表明CCN 2和FGF-1的直接相互作用，并表明这些分子在软骨微环境中共存。CCN 2是软骨发育和再生的众所周知的促进剂，而FGF-1在软骨中的生理和病理作用大多仍不清楚。FGF-1本身在软骨中的生物学作用也被怀疑。
In an attempt to find out a new molecular counterpart of CCN family protein 2 (CCN2), a matricellular protein with multiple functions, we performed an interactome analysis and found fibroblast growth factor (FGF) -1 as one of the candidates. Solid-phase binding assay indicated specific binding between CCN2 and FGF-1. This binding was also confirmed by surface plasmon resonance (SPR) analysis that revealed a dissociation constant (Kd) of 3.98 nM indicating strong molecular interaction between the two. RNA analysis suggested that both FGF-1 and CCN2 could be produced by chondrocytes and thus their interaction in the cartilage is possible. These findings for the first time indicate the direct interaction of CCN2 and FGF-1 and suggest the co-presence of these molecules in the cartilage microenvironment. CCN2 is a well-known promoter of cartilage development and regeneration, whereas the physiological and pathological role of FGF-1 in cartilage mostly remains unclear. Biological role of FGF-1 itself in cartilage is also suspected.