A Fluorescence Detected Magnetic Resonance Investigation of the Carotenoid Triplet States Associated with Photosystem II of Isolated Spinach Thylakoid Membranes
A Fluorescence Detected Magnetic Resonance Investigation of the Carotenoid Triplet States Associated with Photosystem II of Isolated Spinach Thylakoid Membranes
复制标题
与离体菠菜类囊体膜光系统 II 相关的类胡萝卜素三重态的荧光检测磁共振研究
DOI：
10.1007/s11120-005-2840-1
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发表时间：
2005
影响因子：
3.7
通讯作者：
D. Carbonera
中科院分区：
文献类型：
--
作者：
S. Santabarbara;G. Agostini;P. Heathcote;D. Carbonera
利用零场磁共振荧光检测技术研究了菠菜类囊体膜中与光系统II荧光发射叶绿素形式相关的类胡萝卜素三联体群体。在680-690 nm发射范围内收集的光谱已通过全局分析程序拟合。至少有五种不同的类胡萝卜素三重态耦合到终端发射叶绿素形式的PS II，峰值在682 nm，687 nm和692 nm，已被确定。在682 nm处，与外部天线发射形式相关的三重态具有零场分裂参数|D| = 0.0385 cm−1，|E| = 0.00367 cm−1;| D| = 0.0404 cm−1，|E| = 0.00379 cm-1，|D| = 0.0386 cm−1，|E| = 0.00406 cm-1，这与先前报道的分离的LHC II复合物的叶黄素非常相似。因此，在这项工作中记录的FDMR光谱提供了洞察组织的LHC II复合物在未受干扰的环境所代表的类囊体膜。通过监测687和692 nm处的叶绿素发射检测到的额外的类胡萝卜素三联体群体被分配给与内天线复合物结合的类胡萝卜素，因此归因于β-胡萝卜素分子。
The carotenoid triplet populations associated with the fluorescence emission chlorophyll forms of Photosystem II have been investigated in isolated spinach thylakoid membranes by means of fluorescence detected magnetic resonance in zero field (FDMR). The spectra collected in the 680–690 nm emission range, have been fitted by a global analysis procedure. At least five different carotenoid triplet states coupled to the terminal emitting chlorophyll forms of PS II, peaking at 682 nm, 687 nm and 692 nm, have been characterised. The triplets associated with the outer antenna emission forms, at 682 nm, have zero field splitting parameters |D| = 0.0385 cm−1, |E| = 0.00367 cm−1; |D| = 0.0404 cm−1, |E| = 0.00379 cm−1 and |D| = 0.0386 cm−1, |E| = 0.00406 cm−1 which are very similar to those previously reported for the xanthophylls of the isolated LHC II complex. Therefore the FDMR spectra recorded in this work provide insights into the organisation of the LHC II complex in the unperturbed environment represented by thylakoid membranes. The additional carotenoid triplet populations, detected by monitoring the chlorophyll emission at 687 and 692 nm, are assigned to carotenoids bound to inner antenna complexes and hence attributed to β-carotene molecules.