Antioxidant properties of minocycline: neuroprotection in an oxidative stress assay and direct radical-scavenging activity
Antioxidant properties of minocycline: neuroprotection in an oxidative stress assay and direct radical-scavenging activity
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DOI：
10.1111/j.1471-4159.2005.03219.x
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发表时间：
2005-08-01
影响因子：
4.7
通讯作者：
Trauger, JW
中科院分区：
文献类型：
--
作者：
Kraus, RL;Pasieczny, R;Trauger, JW
米诺环素在许多急性CNS损伤和神经退行性疾病的动物模型中具有神经保护作用。虽然米诺环素的抗炎和抗凋亡作用已被表征，但米诺环素神经保护作用的分子基础仍不清楚。我们在这里报告，米诺环素和一些抗氧化剂化合物保护混合神经元文化在氧化应激试验。为了评估米诺环素的直接抗氧化特性在神经保护中的作用，我们使用一组体外自由基清除试验测定了米诺环素、其他四环素类抗生素和参考抗氧化化合物的效力。体外大鼠脑匀浆脂质过氧化和2，2-二苯基-1-苦基肼（DPPH）自由基清除试验的数据表明，与四环素相反，米诺环素是一种有效的抗氧化剂，其自由基清除效力与维生素E相似。我们的研究结果表明，米诺环素的直接抗氧化活性可能有助于其在一些基于细胞的测定和神经元损伤的动物模型中的神经保护作用。
Minocycline is neuroprotective in animal models of a number of acute CNS injuries and neurodegenerative diseases. While anti-inflammatory and anti-apoptotic effects of minocycline have been characterized, the molecular basis for the neuroprotective effects of minocycline remains unclear. We report here that minocycline and a number of antioxidant compounds protect mixed neuronal cultures in an oxidative stress assay. To evaluate the role of minocycline's direct antioxidant properties in neuroprotection, we determined potencies for minocycline, other tetracycline antibiotics, and reference antioxidant compounds using a panel of in vitro radical scavenging assays. Data from in vitro rat brain homogenate lipid peroxidation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays show that minocycline, in contrast to tetracycline, is an effective antioxidant with radical scavenging potency similar to vitamin E. Our findings suggest that the direct antioxidant activity of minocycline may contribute to its neuroprotective effects in some cell-based assays and animal models of neuronal injury.