MONOCLONAL ANTIBODIES AGAINST RABIES VIRUS PRODUCED BY SOMATIC-CELL HYBRIDIZATION - DETECTION OF ANTIGENIC VARIANTS
MONOCLONAL ANTIBODIES AGAINST RABIES VIRUS PRODUCED BY SOMATIC-CELL HYBRIDIZATION - DETECTION OF ANTIGENIC VARIANTS
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DOI：
10.1073/pnas.75.8.3938
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
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作者：
WIKTOR, TJ;KOPROWSKI, H
用灭活狂犬病疫苗免疫BALB/c小鼠，获得骨髓瘤细胞和脾细胞的体细胞杂交瘤，产生抗狂犬病病毒的抗体。具有不同特异性的单克隆抗体可直接从融合后的大量培养物或从单细胞克隆程序衍生的克隆中获得。通过病毒中和程序分析了几种固定或街头狂犬病病毒株，证明其抗原组成存在差异。杂交抗体能够保护实验动物（小鼠）免受致命的狂犬病病毒感染。
Somatic cell hybrids (hybridomas) between mouse myeloma cells and spleen cells derived from BALB/c mice immunized with inactivated rabies vaccine produced antibodies to rabies virus. Monoclonal antibodies with different specificities were obtained from the mass culture directly after fusion or from clones derived from a single-cell cloning procedure. Several strains of fixed or street rabies virus were analyzed by virus neutralization procedures which demonstrated differences in their antigenic composition. Hybridoma antibodies were able to protect experimental animals [mice] from lethal rabies virus infection.