In vitro HPV-11 infection of human foreskin.
In vitro HPV-11 infection of human foreskin.
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人包皮的体外 HPV-11 感染。
DOI：
10.1111/1523-1747.ep12365409
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Isseroff,R
中科院分区：
文献类型：
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作者：
Smith,LH;Foster,C;Hitchcock,ME;Isseroff,R
人类乳头瘤病毒11型(HPV-11)感染的新生人类包皮上皮可以在裸鼠体内以异种移植的形式增殖为尖锐湿疣样生长，这一发现促进了对HPV-11感染过程的研究。在这里，我们描述了用聚合酶链式反应和跨越最流行的早期基因HPV-11转录本(E1∧E4)剪接供受部位的引物检测体外感染和培养的新生儿包皮源性角质形成细胞HPV11感染情况。60℃的热灭活可使E1HPVE4HPV-11mRNA的表达被取消。HPV-11感染的包皮外植体在培养5周内继续产生E1∧E4mRNA，从感染的外植体长出的第二代角质形成细胞继续产生E1∧E4mRNA。本文介绍的体外培养系统为研究HPV-11感染提供了一种新的方法，并可能有助于评估感染的早期事件。
Study of the infectious process of human papillomavirus type 11 (HPV-11) has been facilitated by the discovery that HPV- 11 – infected neonatal human foreskin epithelium can proliferate as xenografts into condyloma-like growths within athymic nude mice. Here we describe detection of HPV-1 1 infection of neonatal human foreskin-derived keratinocytes, infected and cultured entirelyin vitro, by use of the polymerase chain reaction and primers straddling the splice donor/ acceptor site of the most prevalent early gene HPV-11 transcript (E1∧E4). Expression of the E1∧E4 HPV-11 mRNA is abrogated by 60°C heat inactivation of the inoculum. HPV- 11 – infected foreskin explants continue to produce the E1∧E4 mRNA for up to 5 weeks in culture, and second-passage keratinocytes derived from infected explant outgrowths continue to produce the E1∧E4 mRNA. Thein vitrosystem described here provides a new way to study HPV-11 infection and may be useful in evaluating early events of infection.