ANTIBODY AGAINST HUMAN ALPHA-1-BETA-1 INTEGRIN INHIBITS HELA-CELL ADHESION TO LAMININ AND TO TYPE-I, TYPE-IV, AND TYPE-V COLLAGENS
ANTIBODY AGAINST HUMAN ALPHA-1-BETA-1 INTEGRIN INHIBITS HELA-CELL ADHESION TO LAMININ AND TO TYPE-I, TYPE-IV, AND TYPE-V COLLAGENS
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DOI：
10.1006/bbrc.1995.1490
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发表时间：
1995-04-06
影响因子：
3.1
通讯作者：
HEINO, J
中科院分区：
文献类型：
--
作者：
RIIKONEN, T;VIHINEN, P;HEINO, J
在HeLa细胞中，β 1整联蛋白与α 1、α 2、α 3、α 5和α 6整联蛋白亚基形成异二聚体。还可以检测到整合素α v β 5。单克隆抗体SR-84在免疫沉淀试验中鉴定了α 1整联蛋白亚基，并抑制α 1相关细胞与不同基质蛋白、层粘连蛋白-1和I型、IV型和V型胶原的粘附，而其对II型胶原粘附的影响是微不足道的。HeLa细胞不附着于VI型胶原。镁的存在对于HeLa细胞粘附是必不可少的，而单独的钙是不够的，高浓度的钙甚至抵消了镁的作用。细胞粘附到I型胶原蛋白是敏感的pH值的变化，不像细胞粘附到IV型胶原蛋白。我们得出的结论是，SR-84是研究α 1整合素相关功能的有价值的工具，并且在HeLa细胞中，α 1 β 1整合素是I型、IV型和V型胶原蛋白的镁依赖性受体，但不适用于II型和VI型胶原蛋白。(C)出版社：Academic Press
In HeLa cells beta 1 integrin forms heterodimers with alpha 1, alpha 2, alpha 3, alpha 5 and alpha 6 integrin subunits. Integrin alpha v beta 5 can also be detected. A monoclonal antibody SR-84 identified the alpha 1 integrin subunit in immunoprecipitation assays and inhibited alpha 1-related cell adhesion to different matrix proteins, laminin-l and type I, IV, and V collagens, whereas its effect on adhesion to type II collagen was marginal. HeLa cells do not attach to type VI collagen. The presence of magnesium was essential for HeLa cell adhesion, whereas calcium alone was not sufficient and high concentrations of calcium even counteracted the effect of magnesium. Cell adhesion to type I collagen was sensitive to changes in pH, unlike cell adhesion to type IV collagen. We conclude that SR-84 is a valuable tool to study alpha 1 integrin-related functions, and that in HeLa cells alpha 1 beta 1 integrin is a magnesium-dependent receptor for type I, IV, and V collagens but not for type II and VI collagens. (C) 1995 Academic Press, Inc.