Long non-coding RNA NEF inhibits proliferation and promotes apoptosis of laryngeal squamous cell carcinoma cells by inhibiting Wnt/-catenin signaling
Long non-coding RNA NEF inhibits proliferation and promotes apoptosis of laryngeal squamous cell carcinoma cells by inhibiting Wnt/-catenin signaling
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DOI：
10.3892/ol.2019.10150
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发表时间：
2019-06-01
期刊：
影响因子：
2.9
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Cui, Xiangyan;Fang, Ning;Wang, Xin
本研究旨在探讨新近发现的FOXA 2长链非编码RNA邻近增强子（lncRNA NEF）在喉鳞状细胞癌（LSCC）中的作用。本研究采用逆转录-定量聚合酶链反应（RT-qPCR）方法检测肿瘤组织及配对正常组织中lncRNA NEF的表达水平。还使用RT-qPCR测量了LSCC患者和健康对照者的血清NEF水平。回顾性分析喉鳞癌患者的临床及随访资料。分别采用受试者工作特征曲线和生存曲线分析评价血清NEF的诊断价值和预后价值。此外，构建NEF表达载体并转染人喉鳞状细胞癌细胞。采用Cell Counting kit-8细胞增殖实验、MTT法和Western blotting法检测NEF过表达对细胞增殖、凋亡和β-catenin表达的影响。与癌旁正常组织相比，喉鳞癌组织中NEF表达明显下调。喉鳞癌患者血清NEF水平显著低于健康对照组。低血清NEF水平可将喉鳞癌患者与健康对照者区分开来，也表明术后生存期较短。NEF过表达可抑制喉鳞癌细胞增殖，促进细胞凋亡，并下调β-catenin的表达. Wnt激动剂对NEF表达没有显著影响;然而，Wnt激动剂降低了NEF过表达对癌细胞增殖和凋亡的影响。总之，lncRNA NEF可能通过抑制Wnt/-catenin信号通路来抑制LSCC细胞的增殖并促进细胞凋亡。
The present study aimed to investigate the involvement of the recently identified long non-coding RNA neighboring enhancer of FOXA2 (lncRNA NEF) in laryngeal squamous cell carcinoma (LSCC). In this study, the expression levels of lncRNA NEF in tumor tissues and paired adjacent normal tissues were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Serum levels of NEF in patients with LSCC and healthy controls were also measured using RT-qPCR. Clinical and follow-up data of patients with LSCC were retrospectively analyzed. Diagnostic and prognostic values of serum NEF were evaluated by receiver operating characteristic curve and survival curve analysis, respectively. In addition, a NEF expression vector was constructed and transfected into human LSCC cells. The effects of NEF overexpression on cell proliferation, apoptosis and -catenin expression were explored by Cell Counting kit-8 cell proliferation assay, MTT assay and western blotting. NEF was significantly downregulated in tumor tissues compared with in paired adjacent normal tissues of patients with LSCC. Serum levels of NEF were significantly lower in patients with LSCC than in healthy controls. Low serum levels of NEF distinguished patients with LSCC from healthy controls, and also indicated shorter postoperative survival. NEF overexpression inhibited proliferation and promoted apoptosis of LSCC cells, and also downregulated -catenin expression. No significant effects of Wnt agonist on NEF expression were identified; however, Wnt agonist reduced the effects of NEF overexpression on cancer cell proliferation and apoptosis. In conclusion, lncRNA NEF may inhibit proliferation and promote apoptosis of LSCC cells by inhibiting Wnt/-catenin signaling.