Comparison of etiological and immunological characteristics of two attenuated Erysipelothrix rhusiopathiae strains of serotypes 1a and 2.
Comparison of etiological and immunological characteristics of two attenuated Erysipelothrix rhusiopathiae strains of serotypes 1a and 2.
复制标题
两种血清型1a和2型猪红斑丹毒丝菌减毒株的病原学和免疫学特征的比较。
DOI：
10.1292/jvms.55.595
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
I. Takahashi
中科院分区：
文献类型：
--
作者：
M. Watarai;T. Sawada;M. Nakagomi;H. Amao;T. Yoshida;I. Takahashi
对两株抗吖啶黄弱毒猪丹毒丝菌Koganei 65-0.15血清型1a（菌株Kg-1a）和2（菌株Kg-2）进行了比较。生化特性表明，菌株之间的反应相似，略有差异。菌株Kg-2比菌株Kg-1a对吖啶黄染料的抗性更强。对ddY株小鼠的致病性，Kg-2株高于Kg-1a株。C3 H/He和A/J。Kg-1a和Kg-2品系间的临床症状在关节炎发生率（P < 0.05）和仅ddY小鼠的全身症状（P < 0.01）方面观察到显著差异。C3 H/He小鼠对Kg-1a和Kg-2株感染的抵抗力高于ddY和A/J小鼠。在ddY小鼠中，菌株Kg-2的三个培养组分（全培养物：WC、培养滤液：CF和杀死的细胞：KC）比菌株Kg-1a具有更强的保护作用。菌株Kg-2的CF在所有组分中的保护性最强。56 ℃和100 ℃加热或胰蛋白酶处理完全降低了两株WC的保护活性，表明WC的主要保护性抗原是蛋白质。结果表明，两株弱毒株对小鼠的免疫原性和致病性不同。
Two acriflavine-fast attenuated Erysipelothrix rhusiopathiae strains Koganei 65-0.15 of serotype 1a (strain Kg-1a) and 2 (strain Kg-2) were comparatively characterized. Biochemical characterization showed the similar reactions with slight variation between the strains. Strain Kg-2 was more resistant to acriflavine dye than strain Kg-1a. Pathogenicity of strain Kg-2 was higher than strain Kg-1a in mice of strains ddY. C3H/He and A/J. Significant differences of clinical signs between strains Kg-1a and Kg-2 were observed in occurrence of arthritis (P < 0.05) and systemic signs (P < 0.01) of only ddY mice. C3H/He mice was more resistant than ddY and A/J mice to the infection of strains Kg-1a and Kg-2. Three culture fractions, whole culture: WC, culture filtrate: CF and killed cells: KC, of strain Kg-2 were more protective than those of strain Kg-1a in ddY mice. CF of strain Kg-2 was most protective in all fractions. Heating at 56 degrees C and 100 degrees C or treatment with trypsin completely reduced the protective activity of WC of the two strains, indicating that major protective antigens of WC were protein. The present results demonstrated that immunogenicity and pathogenicity for mice were different between the two attenuated strains.